Label-Free Quantitative Proteomic Reveals Differentially Expressed Proteins in Aeromonas-Immunostimulated Leukocytes of Lampetra japonica
Lamprey was considered to be one of the most basal jawless vertebrate representatives for studying vertebrate evolution, embryo development, and the origin of adaptive immunity. Here we investigated the effect of the gut-derived Aeromonas on the lamprey leukocytes proteome using the label-free liquid chromatography–tandem mass spectrometry for quantitative proteomics analysis. Significant difference was observed in the regulation of 34 out of 755 proteins in Aeromonas-immunized lamprey. 31 proteins were only identified in saline solution-immunized lamprey and 47 proteins were only identified in Aeromonas-immunized lamprey. Quantitative real-time polymerase chain reaction was used to validate the results of the proteomic analysis. The differentially expressed proteins were found to be associated with several different biological processes. The identification of leukocytes proteins essential for lamprey adaptive immune response induced by gut-derived Aeromonas strain could supply important information on lamprey–Aeromonas interactions and VLR-based adaptive immune signal pathways.
This study was supported by Natural Science Foundation of China (No. 31500106), the General Scientific Research Foundation of Liaoning Educational Committee (No. L201683674), the Chinese Major State Basic Research Development Program (973 Program; Grant 2013CB835304), and the Chinese National Natural Science Foundation (Grants 31170353). We want to thank Shanghai Applied Protein Technology Co. Ltd for MALDI-TOF MS/MS, database search, and bioinformatic analysis.
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Conflict of interest
The authors declare that they have no conflict of interest.
- 11.Baumgart M, Dogan B, Rishniw M, Weitzman G, Bosworth B, Yantiss R et al (2007) Culture independent analysis of ileal mucosa reveals a selective increase in invasive Escherichia coli of novel phylogeny relative to depletion of Clostridiales in Crohn’s disease involving the ileum. ISME J 1:403–418CrossRefPubMedGoogle Scholar
- 32.Park J-S, Kwok S-K, Lim M-A, Kim E-K, Ryu J-G, Kim S-M et al (2014) STA-21, a promising STAT-3 inhibitor that reciprocally regulates Th17 and Treg cells, inhibits osteoclastogenesis in mice and humans and alleviates autoimmune inflammation in an experimental model of rheumatoid arthritis. Arthritis Rheumatol 66:918–929CrossRefPubMedGoogle Scholar
- 43.Amit I, Yakir L, Katz M, Zwang Y, Marmor MD, Citri A, Shtiegman K, Alroy I, Tuvia S, Reiss Y, Roubini E, Cohen M, Wides R, Bacharach E, Schubert U, Yarden Y (2004) Tal, a tsg101-specific e3 ubiquitin ligase, regulates receptor endocytosis and retrovirus budding. Genes Dev 18(14):1737–1752CrossRefPubMedPubMedCentralGoogle Scholar