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Current Microbiology

, Volume 75, Issue 6, pp 745–751 | Cite as

The Changes of Colonic Bacterial Composition and Bacterial Metabolism Induced by an Early Food Introduction in a Neonatal Porcine Model

  • Chao Shi
  • Yizhi Zhu
  • Qingyan Niu
  • Jue Wang
  • Jing WangEmail author
  • Weiyun Zhu
Article

Abstract

The impact of an early food introduction on the microbiota composition and microbial metabolism in colon was investigated using a new-born piglet model. At day 4 after birth, 10 litters of piglets were randomly allocated to a sow-rearing group (SR group) and a milk-replacer supplementing group (MRS group) (n = 5). A commercial milk replacer was given to the suckling piglets in the MRS group from the 4th day to the 28th day. Pyrosequencing of the V3–V4 region of the 16S rRNA genes showed that the milk replacer supplementation significantly decreased the relative abundance of Lactobacillus, Clostridium XI, Blautia, Clostridium sensustricto and Escherichia (p = 0.08) in the colon of the piglets, but significantly increased the relative abundance of Paraprevotella on the 28th day. In addition, the abundance of Rumminococcus, Clostridium XlVa, Succiniclasticum, Clostridium IV tended to increase in the MRS group. The concentrations of acetate, propionate, butyrate, valerate and branch-chain fatty acids (BCFAs) in the colonic digesta increased with the milk replacer supplementary in the MRS group. In addition, the milk replacer supplementary increased the expression level of Toll-like receptor 4 (TLR4), but decreased the expression level of interleukin-6 (IL-6) in the colonic mucosa of the piglets. In conclusion, an early food introduction can influence the gut bacterial composition and metabolism, and may further affect the intestinal health by modifying the gene transcription related to the colonic function. These findings may provide some guidelines for the early nutrition supplementation for infants during the lactation period.

Keywords

Early food introduction Microbiota Metabolites Gene expression Colon Piglets 

Notes

Acknowledgements

The authors thank National Center for International Research on Animal Gut Nutrition for financial support. This study was supported by National Key R&D Program of China 2017YFD0500505 and the Fundamental Research Funds for the Central Universities, China (KYZ201722).

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no competing interests.

Supplementary material

284_2018_1442_MOESM1_ESM.docx (165 kb)
Supplementary material 1 (DOCX 164 KB)
284_2018_1442_MOESM2_ESM.docx (18 kb)
Supplementary material 2 (DOCX 18 KB)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Chao Shi
    • 1
  • Yizhi Zhu
    • 1
  • Qingyan Niu
    • 1
  • Jue Wang
    • 1
  • Jing Wang
    • 1
    Email author
  • Weiyun Zhu
    • 1
  1. 1.Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, Laboratory of Gastrointestinal Microbiology, College of Animal Science and Technology, National Center for International Research on Animal Gut NutritionNanjing Agricultural UniversityNanjingChina

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