Bacteriophages as Biological Control Agents of Enteric Bacteria Contaminating Edible Oysters
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Bacterial contamination on seafood resulting from unhygienic food-handling practices causes foodborne diseases and significant revenue losses. Moreover, control measures are complicated by a high prevalence of antibiotic-resistant bacteria. Alternative measures such as the phage therapy, therefore, is considered as an environmental and consumer-friendly biological control strategy for controlling such bacterial contamination. In this study, we determined the effectiveness of a bacteriophage cocktail in controlling E. coli strains [JM 109, ATCC 13706 and the, extended spectrum beta-lactamase resistant strain (ATCC BAA 196)] and S. enterica subsp. enterica (ATCC 13311) as single and combined contaminants of the edible oysters. Five different E. coli-specific phages (belonging to the Siphoviridae family) and a Salmonella phage (belonging to the Tectiviridae family) were successfully isolated from sewage water samples taken from a local sewage treatment plan in the Sunshine Coast region of Australia. Phage treatments applied to the pathogens when they were presented on the oysters as either single or combined hosts, resulted in significant decrease of the number of these bacteria on edible oysters. Results obtained indicated that bacteriophages could have beneficial applications in oyster-processing plants in controlling pathogenic bacterial infestations. This study thus contributes towards ongoing international efforts into the effective use of bacteriophages for biological control purposes.
Tuan Son Le gratefully acknowledge MOET-VIED/USC PhD scholarship. Authors thank Mr. Daniel Shelley (University of the Sunshine Coast, Australia) for the technical support provided with the TEM micrographs. Authors thank Dr. Nguyen Hong Nguyen (University of the Sunshine Coast, Australia) for advice on statistical analysis.
Compliance with Ethical Standards
Conflict of interest
The authors declare no conflict of interest.
- 3.Bean NH, Goulding JS, Lao C, Angulo FJ (1996) Surveillance for foodborne-disease outbreaks–United States, 1988–1992. MMWR CDC surveillance summaries: morbidity and mortality weekly report CDC surveillance summaries. 45 (5):1–66Google Scholar
- 4.Bradley S, Anderson D, Jones L (1961) Phylogeny of actinomycetes as revealed by susceptibility to actinophage. Dev Ind Microbiol 2:223–237Google Scholar
- 7.Croci L, Suffredini E (2002) Microbiological risk associated with seafood consumption. Annali dell’Istituto superiore di sanita 39(1):35–45Google Scholar
- 10.Gunathilaka GU (2014) Characterization of bacteriophages from environmental water samples and the potential of bacteriophages tailspike proteins (tsp) in bacteria detection. Wayne State University Theses 300Google Scholar
- 12.Hudson J (2011) Minimum growth temperatures of foodborne pathogens and recommended chiller temperatures. Client Report FW1104 A report for MAF Food Safety ESRGoogle Scholar
- 15.Jan A, Bhat K, Bhat S, Mir M, Bhat M, Imtiyaz A, Rather J (2013) Surface sterilization method for reducing microbial contamination of field grown strawberry explants intended for in vitro culture. Afr J Biotechnol 12:(39)Google Scholar
- 18.Kulikov EE, Golomidova AK, Letarova MA, Kostryukova ES, Zelenin AS, Prokhorov NS, Letarov AV (2014) Genomic sequencing and biological characteristics of a novel Escherichia coli bacteriophage 9 g, a putative representative of a new Siphoviridae genus. Viruses 6(12):5077–5092CrossRefPubMedPubMedCentralGoogle Scholar
- 24.Mazzocco A, Waddell TE, Lingohr E, Johnson RP (2009) Enumeration of bacteriophages by the direct plating plaque assay. Mol Biol 501:77–80Google Scholar
- 25.Moineau S, Pandian S, Klaenhammer TR (1994) Evolution of a lytic bacteriophage via DNA acquisition from the Lactococcus lactis chromosome. Appl Environ Microb 60(6):1832–1841Google Scholar
- 27.NSW Food Authority (2009) Microbiological quality guide for ready-to-eat foods: A guide to interpreting microbiological results. NSW/FA/CP028/0906:1-9Google Scholar
- 29.Savage J, Hobsbawn P (2014) Australian fisheries and aquaculture statistics 2014. Fisheries Research and Development Corporation Project 245Google Scholar