Abstract
A novel virulent bacteriophage, vB_PmuP_PHB02 (phage PHB02), infecting Pasteurella multocida capsular type A strains, was isolated from wastewater from a swine farm in China. Phage PHB02 has a linear double-stranded DNA genome consisting of 38,670 base pairs (bp), with a G+C content of 40.8% and a 127-bp terminal redundancy. Forty-eight putative open reading frames were identified, and no transfer RNA-encoding genes were detected. The morphology and genomic structure of phage PHB02 resemble those of T7-like phages belonging to the family Podoviridae, of the order Caudovirales. Phage PHB02 was stable over a wide range of temperatures (4–50 °C) and pH values (5.0–9.0), and lysed 30 of the 31 capsular-type-A P. multocida strains tested. Phage PHB02 had no effect on other bacterial species or on P. multocida strains belonging to capsular types D or F.
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This study was funded by Research projects of agricultural public welfare industry of China (201403054) and National Science supported planning projects of Hubei Province of China (2014BBB010).
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Chen, Y., Sun, E., Song, J. et al. Complete Genome Sequence of a Novel T7-Like Bacteriophage from a Pasteurella multocida Capsular Type A Isolate. Curr Microbiol 75, 574–579 (2018). https://doi.org/10.1007/s00284-017-1419-3
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DOI: https://doi.org/10.1007/s00284-017-1419-3