Abstract
Expression of genes required for natural genetic competence in Staphylococcus aureus is controlled by an alternative transcription sigma factor, SigH. However, even in the SigH-expressing cells, the DNA transformation efficiency varies depending on culture conditions. We report here that cells grown in the competence-inducing medium (CS2 medium) exhibit enlarged morphology with disintegrated cell walls. Notably, an autolysis inhibitor, Sodium Polyanethol Sulfonate (SPS), facilitated transformation in CS2 medium in a dose-dependent manner, suggesting the involvement of the cell wall metabolism in transformation. However, the transformation efficiency of cells grown in TSB was not improved by physical or enzymatic damage on the cell walls.
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References
Cafini F, Romero VM, Morikawa K (2017) Mechanisms of horizontal gene transfer, in The rise of virulence and antibiotic resistance in Staphylococcus aureus. InTech
Morikawa K, Inose Y, Okamura H, Maruyama A, Hayashi H, Takeyasu K et al (2003) A new staphylococcal sigma factor in the conserved gene cassette: functional significance and implication for the evolutionary processes. Genes Cells 8:699–712
Morikawa K, Takemura AJ, Inose Y, Tsai M, Nguyen Thi le T, Ohta T et al (2012) Expression of a cryptic secondary sigma factor gene unveils natural competence for DNA transformation in Staphylococcus aureus. PLoS Pathog 8:e1003003
Nguyen Thi le T, Romero VM, Morikawa K (2016) Cell wall-affecting antibiotics modulate natural transformation in SigH-expressing Staphylococcus aureus. J Antibiot 69:464–466
Anderson RC, Haverkamp RG, Yu PL (2004) Investigation of morphological changes to Staphylococcus aureus induced by ovine-derived antimicrobial peptides using TEM and AFM. FEMS Microbiol Lett 240:105–110
Mani N, Tobin P, Jayaswal RK (1993) Isolation and characterization of autolysis-defective mutants of Staphylococcus aureus created by Tn917-lacZ mutagenesis. J Bacteriol 175:1493–1499
Sugai M, Akiyama T, Komatsuzawa H, Miyake Y, Suginaka H (1990) Characterization of sodium dodecyl sulfate-stable Staphylococcus aureus bacteriolytic enzymes by polyacrylamide gel electrophoresis. J Bacteriol 172:6494–6498
Qoronfleh MW, Wilkinson BJ (1986) Effects of growth of methicillin-resistant and -susceptible Staphylococcus aureus in the presence of beta-lactams on peptidoglycan structure and susceptibility to lytic enzymes. Antimicrob Agents Chemother 29:250–257
Wecke J, Lahav M, Ginsburg I, Kwa E, Giesbrecht P (1986) Inhibition of wall autolysis of staphylococci by sodium polyanethole sulfonate “liquoid”. Arch Microbiol 144:110–115
Dubrac S, Boneca IG, Poupel O, Msadek T (2007) New insights into the WalK/WalR (YycG/YycF) essential signal transduction pathway reveal a major role in controlling cell wall metabolism and biofilm formation in Staphylococcus aureus. J Bacteriol 189:8257–8269
Kumar JK (2008) Lysostaphin: an antistaphylococcal agent. Appl Microbiol Biotechnol 80:555–561
Johnston C, Martin B, Fichant G, Polard P, Claverys JP (2014) Bacterial transformation: distribution, shared mechanisms and divergent control. Nat Rev Microbiol 12:181–196
Fagerlund A, Granum PE, Havarstein LS (2014) Staphylococcus aureus competence genes: mapping of the SigH, ComK1 and ComK2 regulons by transcriptome sequencing. Mol Microbiol 94:557–579
Briley K Jr, Prepiak P, Dias MJ, Hahn J, Dubnau D (2011) Maf acts downstream of ComGA to arrest cell division in competent cells of B. subtilis. Mol Microbiol 81:23–39
Hahn J, Tanner AW, Carabetta VJ, Cristea IM, Dubnau D (2015) ComGA-RelA interaction and persistence in the Bacillus subtilis K-state. Mol Microbiol 97:454–471
Bayles KW (2007) The biological role of death and lysis in biofilm development. Nat Rev Microbiol 5:721–726
Ranhand JM (1973) Autolytic activity and its association with the development of competence in group H streptococci. J Bacteriol 115:607–614
van der Kooi-Pol MM, Reilman E, Sibbald MJ, Veenstra-Kyuchukova YK, Kouwen TR, Buist G et al (2012) Requirement of signal peptidase ComC and thiol-disulfide oxidoreductase DsbA for optimal cell surface display of pseudopilin ComGC in Staphylococcus aureus. Appl Environ Microbiol 78:7124–7127
Berge MJ, Kamgoue A, Martin B, Polard P, Campo N, Claverys JP (2013) Midcell recruitment of the DNA uptake and virulence nuclease, EndA, for pneumococcal transformation. PLoS Pathog 9:e1003596
Acknowledgements
We thank Ms. Junko Sakamoto for electron microscopy. This work was supported by Takeda Science Foundation, Pfizer Academic Contributions, and The Waksman Foundation of Japan.
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Nguyen, L.T.T., Takemura, A.J., Ohniwa, R.L. et al. Sodium Polyanethol Sulfonate Modulates Natural Transformation of SigH-Expressing Staphylococcus aureus . Curr Microbiol 75, 499–504 (2018). https://doi.org/10.1007/s00284-017-1409-5
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DOI: https://doi.org/10.1007/s00284-017-1409-5