Phylogeny and Antagonistic Activities of Culturable Bacteria Associated with the Gut Microbiota of the Sea Urchin (Paracentrotus lividus)
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In this study, we have investigated the phylogeny and the antagonistic interactions of culturable bacteria isolated from the sea urchin Paracentrotus lividus collected from Aber and Morgat, both located in Crozon peninsula, France. Bacteria were isolated from the gastrointestinal tracts of ten specimens by using conventional culture-dependent method and then investigated by using phylogenetic analysis based on 16S rRNA gene sequence comparisons. Assays for antagonistic interactions among the bacterial strains were performed; bacteria (including at least one strain representative of each OTU identified) were screened for antimicrobial substance production. So, 367 bacterial strains were isolated on marine-agar. On the basis of morphological characteristics, 180 strains were sequenced and 94 OTUs were classified. The dominant phyla were Proteobacteria, Firmicutes and Actinobacteria, with a high abundance of the strains belonging to the genus Psychrobacter. From the antagonistic interactions assays, it could be determined that 22.7% strains were positive for at least one antagonism interaction, 18.3% of them isolated from the sea urchins collected in Morgat. We hypothesize that the bacteria isolated in this study may represent the transitory microbiota of the gastrointestinal tract of P. lividus, and that this microbiota may be related to the diet of this marine invertebrate. Furthermore, our results suggest that chemical antagonism could play a significant role in shaping the bacterial communities within gastrointestinal tract of the sea urchins. In addition, most isolated bacteria may have promising biotechnology applications.
KeywordsAntimicrobials Biotechnology Culture-dependent approach Gut microbiome Paracentrotus lividus Psychrobacter
This work was supported by the National Council for Scientific and Technological Development (CNPq), the National Council for the Improvement of Higher Education (CAPES), the Carlos Chagas Filho Foundation for Research Support of Rio de Janeiro State (FAPERJ) to Laport, MS and by a “Crédit de Recherches” grant from the Fonds de la Recherche Scientifique (FRS-FNRS) to George, I. We are also grateful to Science Without Borders, a CNPq Program for the post doctorate scholarship to Laport, MS; and to Prof. Philippe Dubois and Prof. Chantal de Ridder for accepting her in the “Laboratoire de Biologie Marine”, at “Université Libre de Bruxelles”, Belgium.
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Conflict of interest
No conflict of interest is declared.
- 5.Benson AK, Kelly SA, Legge R, Ma F, Low SJ, Kim J et al (2010) Individuality in gut microbiota composition is a complex polygenic trait shaped by multiple environmental and host genetic factors. Proc Natl Acad Sci USA 107:18933–18938. https://doi.org/10.1073/pnas.1007028107 CrossRefPubMedPubMedCentralGoogle Scholar
- 13.De Filippo C, Cavalieri D, Di Paola M, Ramazzotti M, Poullet JB, Massart S et al (2010) Impact of diet in shaping gut microbiota revealed by a comparative study in children from Europe and rural Africa. Proc Natl Acad Sci USA 107:14691–14696. https://doi.org/10.1073/pnas.1005963107 CrossRefPubMedPubMedCentralGoogle Scholar
- 14.De Ridder C, Foret TW (2001) Non-parasitic symbioses between echinoderms and bacteria. In: Jangoux M, Lawrence JM (eds) Echinoderm studies. AA Balkema, Rotterdam, pp 111–169Google Scholar
- 15.Flemer B, Kennedy J, Margassery LM, Morrissey JP, O’Gara F, Dobson AD (2011) Diversity and antimicrobial activities of microbes from two Irish marine sponges, Suberitescarnosus and Leucosolenia sp. J Appl Microbiol 112:289–301. https://doi.org/10.1111/j.1365-2672.2011.05211.x CrossRefPubMedGoogle Scholar
- 20.Laport MS, Santos-Gandelman JF, Muricy G, Giambiade-deMarval M, George I (2016) Antagonistic interactions among bacteria isolated from either the same or from different sponges native to the Brazilian coast. J Marine Sci Res Dev 6:185. https://doi.org/10.4172/2155-9910.1000185 CrossRefGoogle Scholar
- 21.Laport MS, Bauwens M, Nunes SO, Willenz P, George I, Muricy G (2017) Culturable bacterial communities associated to Brazilian Oscarella species (Porifera: Homoscleromorpha) and their antagonistic interactions. Antonie Van Leeuwenhoek 110:489–499. https://doi.org/10.1007/s10482-016-0818-y CrossRefPubMedGoogle Scholar
- 25.Marinho PR, Moreira AP, Pellegrino FL, Muricy G, Bastos MC, Santos KR et al (2009) Marine Pseudomonas putida: a potential source of antimicrobial substances against antibiotic-resistant bacteria. MIOC 104:678–682Google Scholar
- 26.Martín-Rodríguez AJ, González-Orive A, Hernández-Creus A, Morales A, Dorta-Guerra R, Norte M et al (2014) On the influence of the culture conditions in bacterial antifouling bioassays and biofilm properties: Shewanella algae, a case study. BMC Microbiol 14:102. https://doi.org/10.1186/1471-2180-14-102 CrossRefPubMedPubMedCentralGoogle Scholar
- 27.Mayer LM, Jumars PA, Bock MJ, Vetter YA, Schmidt JL (2001) Two roads to sparagmos: extracellular digestion of sedimentary food by bacterial inoculation versus deposit feeding. In: Aller JY, Woodin SA, Aller RC (eds) Organism-sediment interactions. University of South Carolina, Columbia, pp 335–347Google Scholar
- 30.Muraoka DD (1990) Managing the sea urchin fishery: an economic perspective. Nat Resour J 30:139Google Scholar
- 37.Rua CP, Trindade-Silva AE, Appolinario LR, Venas TM, Garcia GD, Carvalho LS et al (2014) Diversity and antimicrobial potential of culturable heterotrophic bacteria associated with the endemic marine sponge Arenosclera brasiliensis. Peer J 2:e419. https://doi.org/10.7717/peerj.419 CrossRefPubMedPubMedCentralGoogle Scholar
- 38.Ruppert EE, Barnes RD (1997) Invertebrate zoology, 6th edn. Saunders College Publishing, OrlandoGoogle Scholar