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Current Microbiology

, Volume 75, Issue 3, pp 284–287 | Cite as

Draft Genome Sequence of Cyclohexylamine-Degrading Strain Acinetobacter sp. YT-02 Isolated

  • Da-Zhong Yan
  • Ya-Ting Gan
  • Hui Zhou
  • Jun Liu
  • Xin Li
Article

Abstract

Acinetobacter sp. YT-02, a Gram-negative bacterium isolated from the activated sludge from a sodium N-cyclohexylsulfamate production plant, has the ability to degrade cyclohexylamine. It was classified as a member of Acinetobacter sp., a Gram-negative bacterium, sharing a 16S rRNA gene sequence identity of 99% with Acinetobacter guangdongensis strain 1NM-4. It could degrade 10 mmol/L cyclohexylamine within 22 h. Based on the identified metabolite, the metabolic pathway of cyclohexylamine could be postulated as it was degraded via cyclohexanone. Draft genome sequence of this strain (2,993, 647 bp of chromosome length) is presented here. We further identified the genes encoding the enzymes involved in cyclohexylamine oxidation to cyclohexanone and the subsequent downstream metabolic pathway of cyclohexanone oxidation. Strain YT-02 has the potentiality to be applied in the treatment of the pollutant cyclohexylamine, and it could also be treated as a research material to study the degradation mechanism of cyclohexylamine.

Notes

Acknowledgements

The study was supported by the National Natural Science Foundation Program of China (31270112) and State Key Laboratory of Microbial Metabolism (Shanghai Jiao Tong University) (MMLKF14-06).

Supplementary material

284_2017_1377_MOESM1_ESM.doc (44 kb)
Supplementary material 1 (DOC 43 KB)

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Copyright information

© Springer Science+Business Media, LLC 2017

Authors and Affiliations

  • Da-Zhong Yan
    • 1
  • Ya-Ting Gan
    • 1
  • Hui Zhou
    • 1
  • Jun Liu
    • 1
  • Xin Li
    • 1
  1. 1.School of Biology and Pharmaceutical EngineeringWuhan Polytechnic UniversityWuhanChina

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