Current Microbiology

, Volume 75, Issue 3, pp 278–283 | Cite as

Vibrio hannami sp. nov., Isolated from Seawater

Article
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Abstract

A Gram-reaction negative, aerobic, motile, non-pigmented and rod-shaped bacterium, designated as 168GH5-2-16T, was isolated from seawater Jeju island. Phylogenetic analysis based on 16S rRNA gene sequence comparison revealed that the strain formed a distinct lineage within the genus Vibrio and was most closely related to Vibrio variabilis R-40492T (96.0%). The DNA G+C content was 49.3 mol%. The major polar lipids were phosphatidylethanolamine (PE) and phosphatidylglycerol (PG). The predominant quinone was ubiquinone-8 (Q-8). The major fatty acids were C16:0, summed feature 3 (comprising C16:1 ω7c/C16:1 ω6c) and summed feature 8 (C18:1 ω7c/C18:1 ω6c) supported the affiliation of 168GH5-2-16T to the genus Vibrio. Moreover, the physiological, biochemical, and taxonomic analysis allowed the phenotypic and genotypic differentiation of strain 168GH5-2-16T from the recognized species of the genus Vibrio. Therefore, strain 168GH5-2-16T represents a novel species of the genus Vibrio, for which the name Vibrio hannami sp. nov. is proposed, with the type strain 168GH5-2-16T (=KACC 19277T = DSM105032T).

Keywords

Vibrio hannami 16S rRNA gene sequence Polyphasic taxomony 

Notes

Acknowledgements

This research was supported by a grant from the Marine Biotechnology Program (20170431) funded by the Ministry of Oceans and Fisheries, Korea, and Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2016R1D1A3B03933067), Korea.

Supplementary material

284_2017_1376_MOESM1_ESM.pptx (561 kb)
Fig. S1. Transmission electron micrograph of strain 168GH5-2-16T. Bar, 1 μm. (PPTX 560 KB)
284_2017_1376_MOESM2_ESM.pptx (1.2 mb)
Fig. S2. Two-dimensional TLC of the total polar lipids of strain 168GH5-2-16T. The TLC plate was stained for total polar lipids with 5 % ethanolic molybdophosphoric acid. Abbreviations: PE, Phosphatidylethanolamine; PG, phosphatidylglycerol; PL, unknown phospholipid; APL, unknown aminophospholipids; ALs, unknown aminolipids and Ls, unknown polar lipids. (PPTX 1202 KB)
284_2017_1376_MOESM3_ESM.docx (34 kb)
Supplementary material 3 (DOCX 34 KB)

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Copyright information

© Springer Science+Business Media, LLC 2017

Authors and Affiliations

  1. 1.Department of Biological Sciences and BiotechnologyHannam University Jeonmin-dongDaejeonRepublic of Korea
  2. 2.Department of BiotechnologyHankyong National UniversityKyonggi-doRepublic of Korea

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