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Current Microbiology

, Volume 75, Issue 3, pp 256–265 | Cite as

Isolation and Potential Biocementation of Calcite Precipitation Inducing Bacteria from Colombian Buildings

  • Sandra M. Montaño-Salazar
  • Juan Lizarazo-Marriaga
  • Pedro F. B. Brandão
Article

Abstract

Microbiological induced calcium carbonate or calcite precipitation (MICP) has become a highly researched issue due to its multiple applications in the construction industry, being a promising alternative with a great biotechnological importance. In this work, potential calcite precipitation inducing bacteria were isolated from mortar and concrete samples of different buildings at the National University of Colombia. Eighteen crystal-precipitating strains were recovered in Urea-CaCl2 solid medium. The 16S rRNA gene sequencing identified isolates as Arthrobacter, Psychrobacillus and Rhodococcus genera. It is reported, for the first time, the calcite precipitation by P. psycrodurans and R. qingshengii. Optical microscopy and Scanning Electron Microscopy showed crystals with irregular and spherical shapes, and beige and white colours. Furthermore, crystals formation appeared to be strain-specific. X-Ray diffraction analysis confirmed crystals composition as CaCO3. Biocementation tests showed that MICP treatments of mortar cubes using P. psycrodurans caused an increase in their compressive strength compared to control samples. The positive action of a native MICP strain in mortar blocks biomineralization is shown, which is of great interest and potential for the construction industry.

Notes

Acknowledgements

Financial support to SMMS is thanked to División de Investigación Sede Bogotá (DIB), Grant No. 19380/19381. The Microbiology Laboratories of the Biology and Pharmacy Departments are thanked for allowing access to the microscopy and incubation equipment. Juan Mauricio Lozano Valcarcel from the Materials Engineering laboratories is also thanked for his collaboration in biocementation tests. Finally, we thank Professor Sa-YoulGhim, College of Natural Sciences, Kyungpook National University, Daegu, Korea, for permitting access to strain A. crystallopoietes KNUC403.

Funding

This study was funded by the División de Investigación Sede Bogotá (DIB), Universidad Nacional de Colombia (Grant Number DIB 19380/19381).

Compliance with Ethical Standards

Conflict of interest

Sandra M. Montaño-Salaza, Juan Lizarazo-Marriaga, and Pedro F. B. Brandão declares that they have no conflict of interest.

Ethical Approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

284_2017_1373_MOESM1_ESM.pdf (352 kb)
Supplementary material 1 (PDF 352 KB)

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Copyright information

© Springer Science+Business Media, LLC 2017

Authors and Affiliations

  • Sandra M. Montaño-Salazar
    • 1
  • Juan Lizarazo-Marriaga
    • 2
  • Pedro F. B. Brandão
    • 3
  1. 1.Departamento de Biología, Facultad de CienciasUniversidad Nacional de ColombiaBogotáColombia
  2. 2.Grupo de Investigación en Análisis, Diseño y Materiales – GIES, Facultad de IngenieríaUniversidad Nacional de ColombiaBogotáColombia
  3. 3.Grupo de Estudios para la Remediación y Mitigación de Impactos Negativos al Ambiente (G.E.R.M.I.N.A.), Laboratorio de Microbiología Ambiental y Aplicada, Departamento de Química, Facultad de CienciasUniversidad Nacional de ColombiaBogotáColombia

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