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Spirosoma harenae sp. nov., a Bacterium Isolated from a Sandy Beach

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Abstract

A Gram-stain-negative, non-motile, non-spore-forming, rod-shaped, aerobic bacterium, designated 15J8-9T, was isolated from a sandy beach in Jeju Island, South Korea. The isolate was able to grow between 10 and 30 °C, pH 5–8, and in presence of 0–1% (w/v) NaCl. Based on 16S rRNA gene phylogenetic analysis, the novel strain was closely related to members of the genus Spirosoma (96.1–90.9% similarities) and showed highest sequence similarity to Spirosoma panaciterrae DSM 21099T (96.1%). The G + C content of the genomic DNA of strain 15J8-9T was 45.1 mol%. The isolate contained menaquinone MK-7 as the predominant respiratory quinone, phosphatidylethanolamine as the major polar lipid, and summed feature 3 (C16:1 ω6c/C16:1 ω7c; 28.0%), C16:1 ω5c (23.4%), iso-C15:0 (13.5%), and C16:0 (11.5%) as the major fatty acids that supported the affiliation of strain 15J8-9T to the genus Spirosoma. The isolate could be differentiated clearly from recognized Spirosoma species on the basis of several phenotypic, genotypic and chemotaxonomic features. Therefore, strain 15J8-9T is considered to represent a novel species of the genus the genus Spirosoma, for which the name Spirosoma harenae sp. nov. is proposed. The type strain is 15J8-9T (= KCTC 52030T = JCM 31993T).

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Acknowledgements

This work was supported by the Human Resource Training Program for Regional Innovation and Creativity through the Ministry of Education and National Research Foundation (NRF) of Korea (NRF-2014H1C1A1066929), and the Brain Pool Program of 2016 (Grant 162S-4-3-1727) through the Korean Federation of Science and Technology Societies (KOFST) funded by the Ministry of Science, ICT and Future Planning, Republic of Korea.

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Authors and Affiliations

  1. School of Applied Biosciences, Kyungpook National University, Daegu, 41566, Republic of Korea

    Leonid N. Ten, Nabil Elderiny, Jae-Jin Lee, Seung-Yeol Lee, Sangkyu Park & Hee-Young Jung

  2. Department of Environmental Engineering, Kyungpook National University, Daegu, 41566, Republic of Korea

    Dae Sung Lee

  3. Department of Bio and Environmental Technology, Seoul Women’s University, Seoul, 01797, Republic of Korea

    Myung Kyum Kim

  4. Institute of Plant Medicine, Kyungpook National University, Daegu, 41566, Republic of Korea

    Hee-Young Jung

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  1. Leonid N. Ten
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Ten, L.N., Elderiny, N., Lee, JJ. et al. Spirosoma harenae sp. nov., a Bacterium Isolated from a Sandy Beach. Curr Microbiol 75, 179–185 (2018). https://doi.org/10.1007/s00284-017-1363-2

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