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Extraction of Lactobacillus acidophilus CICC 6074 S-Layer Proteins and Their Ability to Inhibit Enteropathogenic Escherichia coli

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Abstract

An adhesion-related protein of Lactobacillus acidophilus strain CICC 6074 involved in binding to Caco-2 cells and inhibiting Enteropathogenic Escherichia coli (EPEC) was isolated and characterized. The S-layer protein was extracted with 5M LiCl and the active fraction purified by gel filtration (G-75). The S-layer protein was visualized by SDS-PAGE and characterized by estimating the relative molecular weight using mass spectra. The inhibitory effect of L. acidophilus and its S-layer proteins on the ability of EPEC to adhere to cells was explored by using a Caco-2 cell model. The results suggest that the S-layer proteins of L. acidophilus are adhesive in nature and are involved in the competitive exclusion of EPEC from Caco-2 cells.

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Acknowledgements

This work was supported by the Natural Science Funding of China (31471598, 41276121, and 31601487) and of Jiangsu Province (BK20141447), the Natural Science Funding of Zhejiang (LQ16C200002), and the Modern Agricultural Technical System Foundation of China (CARS-43-17).

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Authors and Affiliations

  1. Department of Food Science and Technology, Nanjing Normal University, 122 Ninghai Road, Nanjing, 210097, People’s Republic of China

    Jinye Zhang, Jinjin Gao, Yuxing Guo & Daodong Pan

  2. Department of Food Science and Technology, Ningbo University, 818 Fenghua Road, Ningbo, 315211, People’s Republic of China

    Zhen Wu & Daodong Pan

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  1. Jinye Zhang
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  2. Jinjin Gao
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  3. Yuxing Guo
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Correspondence to Daodong Pan.

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Zhang, J., Gao, J., Guo, Y. et al. Extraction of Lactobacillus acidophilus CICC 6074 S-Layer Proteins and Their Ability to Inhibit Enteropathogenic Escherichia coli . Curr Microbiol 74, 1123–1129 (2017). https://doi.org/10.1007/s00284-017-1291-1

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