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Role of Sod Gene in Response to Static Magnetic Fields in Pseudomonas aeruginosa

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Abstract

The protective role of superoxide dismutase (SOD) against non-ionizing radiation such as static electromagnetic field (200 mT) has been studied in wild-type and mutant strain of Pseudomonas aeruginosa lacking cytosolic Mn-SOD (sodM), Fe-SOD (sodB), or both SODs (sodMB). Our results showed that inactivation of sodM and/or sodB genes increases the sensitivity of P. aeruginosa toward stress induced by the static magnetic field (200 mT). Furthermore, our results showed an enhancement of SOD, catalase, and peroxidases after exposure to the magnetic field. However, wild-type cells maintained significantly higher activities of antioxidant enzymes than mutant strains. The malondialdehyde produced by the oxidative degradation of unsaturated lipids and fatty acids showed significant increase in mutant strains compared to the wild-type. The overall results showed that the SOD has a protective role against a stress induced by static electromagnetic field in P. aeruginosa.

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Acknowledgement

The authors are grateful to Mr Iiyama Kazuhiro of the Fukuoka University of Agriculture for providing bacterial strains.

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Authors and Affiliations

  1. Unité de Biochimie des lipides et interactions des macromolécules en Biologie (03/UR/0902), Laboratoire de Biochimie et biologie moléculaire, Faculté des Sciences de Bizerte, Zarzouna 7021, Bizerte, Tunisia

    Raouia Hanini & Ahmed Landoulsi

  2. Laboratoire de Traitement des Eaux Usées, Centre de Recherches et Technologies des Eaux, Technopole Borj Cedria, Tunis, Tunisia

    Abdelwaheb Chatti & Selma Ben Ghorbel

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  1. Raouia Hanini
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  2. Abdelwaheb Chatti
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  4. Ahmed Landoulsi
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Correspondence to Raouia Hanini.

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Hanini, R., Chatti, A., Ghorbel, S.B. et al. Role of Sod Gene in Response to Static Magnetic Fields in Pseudomonas aeruginosa . Curr Microbiol 74, 930–937 (2017). https://doi.org/10.1007/s00284-017-1264-4

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  • DOI: https://doi.org/10.1007/s00284-017-1264-4

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