Current Microbiology

, Volume 74, Issue 7, pp 885–888 | Cite as

Sucralose Increases Antimicrobial Resistance and Stimulates Recovery of Escherichia coli Mutants

  • Yilin Qu
  • Rongyan Li
  • Mingshan Jiang
  • Xiuhong Wang
Letter To the Editor


Because of heavy use of antimicrobials, antimicrobial resistance in bacteria has become of great concern. The effect of some widely used food additives such as sucralose on bacteria in the gut and the environment has also drawn increasing attention. In this study, we investigated the interaction between antimicrobials and sucralose impacting antimicrobial resistance and mutation of Escherichia coli (E. coli). To examine antimicrobial resistance and mutation frequency, different subinhibitory concentrations of sucralose were added to cultures of E.coli BW25113 that were then treated with antimicrobials, oxolinic acid, or moxifloxacin. Then the E.coli were assayed for bacterial survival and recovery of mutants resistant to an unrelated antimicrobial, rifampicin. Pre-treatment of E.coli BW25113 with 1/2 minimal inhibitory concentration (MIC) of sucralose increased the survival rate in oxolinic acid or moxifloxacin. A 1/3 MIC of sucralose increased rifampicin-resistant mutation rate of E.coli BW25113 after 72 h, while rifampicin-resistant mutation rate was increased when co-treated with 1/8 MIC, 1/4 MIC, 1/3 MIC sucralose, and oxolinic acid after 24 h. Sucralose can increase the antimicrobial resistance and mutation frequency of E.coli to some antimicrobials.


Sucralose Escherichia coli Antimicrobial resistance Mutation frequency 



This study was funded by Youth Science Foundation of Heilongjiang province of China (Grant Number QC2014C090) and the China Postdoctoral Science Foundation (Grant Number 2013T60385).

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  • Yilin Qu
    • 1
  • Rongyan Li
    • 2
  • Mingshan Jiang
    • 3
  • Xiuhong Wang
    • 1
  1. 1.Department of Biochemistry and Molecular BiologyHarbin Medical UniversityHarbinChina
  2. 2.Department of Anesthesiology, Changhai HospitalSecond Military Medical UniversityShanghaiChina
  3. 3.Department of General SurgeryThe 2nd Affiliated Hospital of Harbin Medical UniversityHarbinChina

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