Isolation and Complete Genome Sequence of a Novel Pseudoalteromonas Phage PH357 from the Yangtze River Estuary
Phage PH357, a novel lytic Pseudoalteromonas lipolytica phage belonging to the Myoviridae family was isolated from the Yangtze River estuary. The microbiological characterization demonstrated that phage PH357 is stable from −20 to 60 °C and the optimal pH 7. The one-step growth curve showed a latent period of 20 min, a rise period of 20 min, and the average burst size was about 85 virions per cell. Complete genome of phage PH357 was determined. Genome of phage PH357 consisted of a linear, double-stranded 136,203 bp DNA molecule with 34.58% G + C content, and 242 putative open reading frames (ORFs) without tRNA. All the predicted ORFs were classified into eight functional groups, including DNA replication, regulation and nucleotide metabolism, transcription, translation, phage packaging, phage structure, lysis, host or phage interactions, and hypothetical protein. A phylogenetic analysis showed that phage PH357 had similarity to the previously published Pseudoalteromonas phage PH101 and Vibrio phages. Furthermore, the study of phage PH357 genome will provide useful information for further research on the interaction between phages and their hosts.
KeywordsPhage Genome Major Capsid Protein Yangtze River Estuary Phage PH357 Terminase Subunit
The authors greatly appreciate the officers, crew, and scientific staff onboard the research vessel Dong Fang Hong 2 for facilitating the collection of the seawater samples.
This work was supported by the National Natural Science Foundation of China (NSFC Grant Nos. 31500339, 41676178, 41076088), the National Key Basic Research Program of China (973Program, Grant No: 2013CB429704), China Postdoctoral Science Foundation (Grant Nos. 2015M570612 and 2016T90649), and Fundamental Research Funds for the Central University of Ocean University of China (Grant Nos. 201564010 and 201512008).
Compliance with Ethical Standards
Conflict of interest
The authors declare that they have no conflict of interest regarding this study.
This article does not contain any studies with human participants or animals performed by any of the authors.
- 1.Bai Q, Zhang W, Yang Y, Tang F, Nguyen X, Liu G, Lu C (2013) Characterization and genome sequencing of a novel bacteriophage infecting streptococcus agalactiae with high similarity to a phage from streptococcus pyogenes. Adv Virol 158(8):1733–1741Google Scholar
- 12.Kallies R, Kiesel B, Schmidt M, Kacza J, Ghanem N, Narr A, Zopfi J, Wick LY, Hackermüller J, Harms H, Chatzinotas A (2017) Complete genome sequence of Pseudoalteromonas phage vB_PspS-H40/1 (formerly H40/1) that infects Pseudoalteromonas sp. strain H40 and is used as biological tracer in hydrological transport studies. Stand Genom Sci 12(1):20CrossRefGoogle Scholar
- 24.Senčilo A, Luhtanen AM, Saarijärvi M, Bamford DH, Roine E (2015) Cold-active bacteriophages from the baltic sea ice have diverse genomes and virus–host interactions. Environ Microbiol 17(10):392–400Google Scholar
- 28.Wang W, Li M, Lin H, Wang J, Mao X (2016) The Vibrio parahaemolyticus-infecting bacteriophage qdvp001: genome sequence and endolysin with a modular structure. Adv Virol 161(10):2645–2652Google Scholar
- 33.Yuan L, Cui Z, Wang Y, Guo X, Zhao Y (2014) Complete genome sequence of virulent bacteriophage shou24, which infects foodborne pathogenic vibrio parahaemolyticus. Adv Virol 159(11):3089–3093Google Scholar