Isolation of Viable but Non-culturable Bacteria from Printing and Dyeing Wastewater Bioreactor Based on Resuscitation Promoting Factor
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Printing and dyeing wastewater with high content of organic matters, high colority, and poor biochemical performance is hard to be degraded. In this study, we isolated viable but non-culturable (VBNC) bacteria from printing and dyeing wastewater with the culture media contained resuscitation promoting factor (Rpf) protein secreted by Micrococcus luteus, counted the culturable cells number with the most probable number, sequenced 16S rRNA genes, and performed polymerase chain reaction-denaturing gradient gel electrophoresis. It is obviously that the addition of Rpf in the enrichment culture could promote growth and resuscitation of bacteria in VBNC state to obtain more fastidious bacteria significantly. The identified bacteria were assigned to nine genera in the treatment group, while the two strains of Ochrobactrum anthropi and Microbacterium sp. could not be isolated from the control group. The function of isolated strains was explored and these strains could degrade the dye of Congo red. This study provides a new sight into the further study including the present state, composition, formation mechanism, and recovery mechanism about VBNC bacteria in printing and dyeing wastewater, which would promote to understand bacterial community in printing and dyeing wastewater, and to obtain VBNC bacteria from ecological environment.
KeywordsBioreactor of printing and dyeing wastewater VBNC bacteria Rpf 16S rRNA gene sequence Phylogenetic relationship PCR-DGGE
This research was supported by the Zhejiang Provincial Public Welfare Technology Applied Research Project (2017C33046), Zhejiang Provincial Natural Science Foundation of China (LY13C010002), Shenzhen Basic Research Project (JCYJ20140417113430641, JCYJ20140509174140691 and JCYJ20140417113430732), Foundation of Shenzhen Strategic Emerging Industries Development (JSKF20150831171545604) and Shenzhen Technology development Project (CXZZ20150529144041624).
Compliance with Ethical Standards
Conflict of interest
We declare that we do not have any commercial or associative interest that represents a conflict of interest in connection with the work submitted.
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