Current Microbiology

, Volume 74, Issue 7, pp 815–820 | Cite as

Characterization and Complete Genome Sequence of a Novel Siphoviridae Bacteriophage BS5

  • Xue Meng
  • Min Wang
  • Siyuan You
  • Duobing Wang
  • Yan Li
  • Zhaoyang Liu
  • Yu Gao
  • Lu Liu
  • Yaoyuan Zhang
  • Zhenghao Yan
  • Chunyan Liu
  • Yong Jiang
  • Hongbing Shao
Article

Abstract

A novel Siphoviridae family Phage BS5, which infects Pseudoalteromonas atlantica, was isolated from the surface waters of the Yellow Sea. Morphological study by transmission electron microscopy revealed that the novel phage belongs to Siphoviridae. The complete genome sequence of PBS5 contained a linear, double-strand 39949-bp DNA molecule with a G + C content of 40.6% and 65 putative open reading frames. Twelve conserved domains were detected by BLASTP in NCBI, and of these the functions of 5 were known. The genome was grouped into four modules as follows: phage structure, phage packaging, DNA replication and regulation, and some additional functions.16 S rDNA sequence analysis was also applied to identify the host bacteria. After initial characterization of bacteriophage PBS5, it was found that the optimal pH was 7.0, the optimal temperature was 30 °C, and the burst size was about 95 virions per cell. This information will provide an important benchmark for further research on the interaction between bacteriophages and their hosts.

Keywords

Phage Genomic Siphoviridae family Phage Yellow Sea 

Notes

Acknowledgements

We are grateful to the research vessel Dong Fang Hong 2, for providing the seawater samples. The research was funded by the National Natural Science Foundation of China (No. 41076088 and 31500339) and China Postdoctoral Science Foundation (No.2015M5Z0612).

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no competing interests.

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  1. 1.College of Marine Life SciencesOcean University of ChinaQingdaoChina
  2. 2.Institute of Evolution and Marine BiodiversityOcean University of ChinaQingdaoChina
  3. 3.No.1 Middle SchoolPingduChina
  4. 4.No.58 Middle SchoolQingdaoChina

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