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Description of Gabonibacter massiliensis gen. nov., sp. nov., a New Member of the Family Porphyromonadaceae Isolated from the Human Gut Microbiota

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Abstract

The identification of human-associated bacteria is very important to control infectious diseases. In recent years, we diversified culture conditions in a strategy named culturomics, and isolated more than 100 new bacterial species and/or genera. Using this strategy, strain GM7, a strictly anaerobic gram-negative bacterium was recently isolated from a stool specimen of a healthy Gabonese patient. It is a motile coccobacillus without catalase and oxidase activities. The genome of Gabonibacter massiliensis is 3,397,022 bp long with 2880 ORFs and a G+C content of 42.09 %. Of the predicted genes, 2,819 are protein-coding genes, and 61 are RNAs. Strain GM7 differs from the closest genera within the family Porphyromonadaceae both genotypically and in shape and motility. Thus, we propose that strain GM7T (=CSUR P2336 = DSM 101039) is the type strain of the new genus Gabonibacter gen. nov. and the new species G. massiliensis gen. nov., sp. nov.

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Abbreviations

CSUR:

Collection de Souches de l’Unité des Rickettsies

DSM:

Deutsche Sammlung von Mikroorganismen

MALDI-TOF MS:

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

URMITE:

Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes

FAME:

Fatty acid methyl ester

GC/MS:

gas chromatography/mass spectrometry

ORF:

Open reading Frame

BMI:

Body mass index

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Acknowledgments

The authors thank the Xegen Company (www.xegen.fr) for automating the genome analysis process and Claudia Andrieu for administrative assistance.

Funding

This project was funded by the Méditerranée-Infection foundation.

Author information

Authors and Affiliations

  1. URMITE, UM63, CNRS 7278, IRD 198, INSERM 1095, IHU Méditerranée-Infection, Faculté de Médecine, Aix-Marseille Université, 27 Bd Jean Moulin, Marseille, France

    Gaël Mourembou, Jaishriram Rathored, Saber Khelaifia, Catherine Robert, Nicholas Armstrong, Didier Raoult & Pierre-Edouard Fournier

  2. Ecole Doctorale Régionale d’Afrique Centrale, B.P. 876, Franceville, Gabon

    Gaël Mourembou

  3. Unité de Parasitologie Médicale (UPARAM), CIRMF B.P. 769, Franceville, Gabon

    Jean Bernard Lekana-Douki

  4. Département de Parasitologie Mycologie et de Médecine Tropicale, Université des Sciences de la Santé, B.P. 4009, Libreville, Gabon

    Jean Bernard Lekana-Douki

  5. Département de Microbiologie, Laboratoire national de référence IST/sida, Faculté de Médecine, Université des Sciences de la Santé, B.P. 4009, Libreville, Gabon

    Angélique Ndjoyi-Mbiguino

  6. Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia

    Didier Raoult

Authors
  1. Gaël Mourembou
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  2. Jaishriram Rathored
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  3. Jean Bernard Lekana-Douki
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  5. Saber Khelaifia
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  6. Catherine Robert
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  7. Nicholas Armstrong
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  8. Didier Raoult
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  9. Pierre-Edouard Fournier
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Corresponding author

Correspondence to Pierre-Edouard Fournier.

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Mourembou, G., Rathored, J., Lekana-Douki, J.B. et al. Description of Gabonibacter massiliensis gen. nov., sp. nov., a New Member of the Family Porphyromonadaceae Isolated from the Human Gut Microbiota. Curr Microbiol 73, 867–877 (2016). https://doi.org/10.1007/s00284-016-1137-2

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