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Effects of Homologous Expression of 1,4-Benzoquinone Reductase and Homogentisate 1,2-Dioxygenase Genes on Wood Decay in Hyper-Lignin-Degrading Fungus Phanerochaete sordida YK-624

Current Microbiology volume 73, pages 512–518 (2016)Cite this article

Abstract

We investigated the function of 1,4-benzoquinone reductase (BQR)- and homogentisate 1,2-dioxygenase (HGD)-like genes in wood degradation by Phanerochaete sordida YK-624, which exhibits high ligninolytic activity and selectivity. We determined homologous expression in the genomic and cDNA sequences of BQR- and HGD-like genes in P. sordida YK-624 (PsBQR and PsHGD). Both genes shared high homology (≥90 % amino acid sequence similarity) with the corresponding genes in Phanerochaete chrysosporium. These genes were co-transformed with a reporter gene into an uracil auxotrophic mutant of P. sordida YK-624. The PsBQR and PsHGD co-transformants exhibited lower holocellulolytic activity and higher ligninolytic selectivity than the control transformants. In liquid culture with vanillin, both co-transformants significantly accelerated vanillin degradation. Thus, we suggest that the rapid metabolism of low-molecular weight lignin fragments, due to the homologous expression of BQR- and HGD-like genes, affects quinone redox cycling to produce hydroxyl radicals, thereby decreasing holocellulose degradation and increasing ligninolytic selectivity.

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Affiliations

  1. Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka, 422-8529, Japan

    Toshio Mori, Genki Koyama, Hirokazu Kawagishi & Hirofumi Hirai

  2. Research Institute of Green Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka, 422-8529, Japan

    Hirokazu Kawagishi & Hirofumi Hirai

  3. Graduate School of Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka, 422-8529, Japan

    Hirokazu Kawagishi

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  1. Toshio Mori
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  2. Genki Koyama
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  3. Hirokazu Kawagishi
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  4. Hirofumi Hirai
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Correspondence to Hirofumi Hirai.

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Mori, T., Koyama, G., Kawagishi, H. et al. Effects of Homologous Expression of 1,4-Benzoquinone Reductase and Homogentisate 1,2-Dioxygenase Genes on Wood Decay in Hyper-Lignin-Degrading Fungus Phanerochaete sordida YK-624. Curr Microbiol 73, 512–518 (2016). https://doi.org/10.1007/s00284-016-1089-6

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  • DOI: https://doi.org/10.1007/s00284-016-1089-6

Keywords

  • Lignin
  • High Performance Liquid Chromatography
  • Vanillin
  • Ligninolytic Enzyme
  • Klason Lignin