A Bilobalide-Producing Endophytic Fungus, Pestalotiopsis uvicola from Medicinal Plant Ginkgo biloba


For screening bilobalide (BB)-producing endophytic fungi from medicinal plant Ginkgo biloba, a total of 57 fungal isolates were isolated from the internal stem, root, leaf, and bark of the plant G. biloba. Fermentation processes using BB-producing fungi other than G. biloba may become a novel way to produce BB, which is a terpene trilactones exhibiting neuroprotective effects. In this study, a BB-producing endophytic fungal strain GZUYX13 was isolated from the leaves of G. biloba grown in the campus of Guizhou University, Guiyang city, Guizhou province, China. The strain produced BB when grown in potato dextrose liquid medium. The amount of BB produced by this endophytic fungus was quantified to be 106 μg/L via high-performance liquid chromatography (HPLC), substantially lower than that produced by the host tissue. The fungal BB which was analyzed by thin layer chromatography (TLC) and HPLC was proven to be identical to authentic BB. The strain GZUYX13 was identified as Pestalotiopsis uvicola via morphology and ITS rDNA phylogeny. To the best of our knowledge, this is the first report concerning the isolation and identification of endophytic BB-producing Pestalotiopsis spp. from the host plant, which further proved that endophytic fungi have the potential to produce bioactive compounds.

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This work was funded by the talent introduction plan of the Guizhou University (Grant No. [2014]59), the joint funds plan of the Guizhou province (Grant No. LH [2014]7657), and the grants of the agricultural science and technology foundation of Guizhou province (Grant Nos. NY[2013]3042 and NY[2013]3044) from the Science and Technology Bureau of Guizhou University and the Science and Technology Department of Guizhou province, China. The authors would also like to thank the National Natural Science Foundation of China (Grant No. 31460011) for supporting and funding this research.

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Correspondence to Ji-Chuan Kang.

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Qian, YX., Kang, JC., Luo, YK. et al. A Bilobalide-Producing Endophytic Fungus, Pestalotiopsis uvicola from Medicinal Plant Ginkgo biloba . Curr Microbiol 73, 280–286 (2016). https://doi.org/10.1007/s00284-016-1060-6

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  • Bark
  • Endophytic Fungus
  • Conidiogenous Cell
  • Ginkgo Biloba
  • Shanghai Sangon Biologic Engineer Technology