A Gram stain-negative, strictly aerobic, brown-pigmented, non-motile, rod-shaped, chemoheterotrophic bacterial strain-designated A6F-119T was isolated from a marine sponge (Rhabdastrella sp.). Phylogenetic analyses based on the 16S rRNA gene sequence revealed that the new strain represented a member of the family Flavobacteriaceae of the phylum Bacteroidetes and that it showed highest sequence similarity (93 %) to Tenacibaculum maritimum NBRC 15946T. The strain could be differentiated phenotypically from the recognized members of the family Flavobacteriaceae. The DNA G + C content of strain A6F-119T was determined to be 30.8 mol%; MK-6 was identified as the major menaquinone; and the presence of iso-C15:0, iso-C17:0 3-OH, and C16:1 ω7c and/or C16:1 ω6c as the major (>10 %) cellular fatty acids. A polar lipid profile was present consisting of phosphatidylethanolamine, an unidentified aminolipid, and three unidentified lipids. From the distinct phylogenetic position and combination of genotypic and phenotypic characteristics, the strain is considered to represent a novel genus for which the name Spongiiferula fulva gen. nov., sp. nov. is proposed. The type strain of S. fulva is A6F-119T (= KCTC 42752T = NBRC 111402T)
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Bernardet JF, Nakagawa Y (2003) An introduction to the family Flavobacteriaceae. In Dworkin M, Falkow S, Rosenberg E, Schleifer KH, Stackebrandt E (eds) The prokaryotes, an evolving electronic resource for the microbiological community, release 3.15. Springer, New York. http://link.springer-ny.com/link/service/books/10125/
Bernardet JF, Nakagawa Y, Holmes B (2002) Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52:1049–1070
Collins MD, Jones D (1981) A note on the separation of natural mixtures of bacterial ubiquinones using reverse-phase partition thin-layer chromatography and high performance liquid chromatography. J Appl Bacteriol 51:129–134
Collins CH, Lyne PM (1984) Microbiological methods, 5th edn. Butterworth, London
DeLong EF, Franks DG, Alldredge AL (1993) Phylogenetic diversity of aggregate-attached vs. free-living marine bacterial assemblages. Limnol Oceanogr 38:924–934
Dittmer JC, Lester RL (1964) A simple, specific spray for the detection of phospholipids on thin-layer chromoatograms. J Lipid Res 15:126–127
Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791
Fitch WM (1971) Towards defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416
Fukui Y, Abe M, Kobayashi M, Saito H, Oikawa H, Yano Y, Satomi M (2013) Polaribacter porphyrae sp. nov., isolated from the red alga Porphyra yezoensis, and emended descriptions of the genus Polaribacter and two Polaribacter species. Int J Syst Evol Microbiol 63:1665–1672
Garrity GM, Holt JG (2001) The road map to the manual. In: Boone DR, Castenholz RW, Garrity GM (eds) Bergey’s manual of systematic bacteriology, vol 1, 2nd edn. Springer, New York, pp 119–166
Glöckner FO, Fuchs BM, Amann R (1999) Bacterioplankton composition of lakes and oceans: a first comparison based on fluorescence in situ hybridization. Appl Environ Microbiol 65:3721–3726
Gosink JJ, Woese CR, Staley JT (1998) Polaribacter gen. nov., with three new species, P. irgensii sp. nov., P. franzmannii sp. nov. and P. filamentus sp. nov., gas vacuolate polar marine bacteria of the Cytophaga-Flavobacterium-Bacteroides group and reclassification of ‘Flectobacillus glomeratus’ as Polaribacter glomeratus comb. nov. Int J Syst Bacteriol 48:223–235
Hansen GH, Sørheim R (1991) Improved method for phenotypical characterization of marine bacteria. J Microbiol Methods 13:231–241
Hyun DW, Shin NR, Kim MS, Kim PS, Jung MJ, Kim JY, Whon TW, Bae JW (2014) Polaribacter atrinae sp. nov., isolated from the intestine of a comb pen shell, Atrina pectinata. Int J Syst Evol Microbiol 64:1654–1661
Jooste PJ (1985) The taxonomy and significance of Flavobacterium–Cytophaga strains from dairy sources. PhD thesis, University of the Orange Free State, South Africa
Khan ST, Nakagawa Y, Harayama S (2008) Fulvibacter tottoriensis gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from marine sediment. Int J Syst Evol Microbiol 58:1670–1674
Kim OS, Cho YJ, Lee K, Yoon SH, Kim M, Na H, Park SC, Jeon YS, Lee JH, Yi H, Won S, Chun J (2012) Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721
Kimura M (1983) The neutral theory of molecular evolution. Cambridge University Press, Cambridge
Kirchman DL (2002) The ecology of Cytophaga-Flavobacteria in aquatic environments. FEMS Microbiol Ecol 39:91–100
Komagata K, Suzuki K (1987) Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207
Lee YS, Baik KS, Park SY, Kim EM, Lee DH, Kahng HY, Jeon CO, Jung JS (2009) Tenacibaculum crassostreae sp. nov., isolated from the Pacific oyster, Crassostrea gigas. Int J Syst Evol Microbiol 59:1609–1614
Li Y, Wei J, Yang C, Lai Q, Chen Z, Li D, Zhang H, Tian Y, Zheng W, Zheng T (2013) Tenacibaculum xiamenense sp. nov., an algicidal bacterium isolated from coastal seawater. Int J Syst Evol Microbiol 63:3481–3486
Li H, Zhang XY, Liu C, Lin CY, Xu Z, Chen XL, Zhou BC, Shi M, Zhang YZ (2014) Polaribacter huanghezhanensis sp. nov., isolated from Arctic fjord sediment, and emended description of the genus Polaribacter. Int J Syst Evol Microbiol 64:973–978
Ludwig W, Klenk HP (2001) Overview: a phylogenetic backbone and taxonomic framework for procaryotic systematics. In: Boone DR, Castenholz RW, Garrity GM (eds) Bergey’s manual of systematic bacteriology, vol 1, 2nd edn. Springer, New York, pp 49–66
Madsen L, Møller JD, Dalsgaard I (2005) Flavobacterium psychrophilum in rainbow trout, Oncorhynchus mykiss (Walbaum), hatcheries: studies on broodstock, eggs, fry and environment. J Fish Dis 28:39–47
Malik YS, Olsen K, Kumar K, Goyal SM (2003) In vitro antibiotic resistance profiles of Ornithobacterium rhinotracheale strains from Minnesota turkeys during 1996–2002. Avian Dis 47:588–593
Marmur J (1961) A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 3:208–218
Mesbah M, Premachandran U, Whitman WB (1989) Precise measurement of the G + C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167
Minnikin DE, O’Donnell AG, Goodfellow M, Alderson G, Athalye M, Schaal A, Parlett JH (1984) An integrated procedure for the extraction of bacterial isoprenoid quinines and polar lipids. J Microbiol Meth 2:233–241
Nedashkovskaya OI, Kukhlevskiy AD, Zhukova NV (2013) Polaribacter reichenbachii sp. nov.: a new marine bacterium associated with the green alga Ulva fenestrata. Curr Microbiol 66:16–21
Nematollahi A, Decostere A, Pasmans F, Haesebrouck F (2003) Flavobacterium psychrophilum infections in salmonid fish. J Fish Dis 26:563–574
Oh YS, Kahng HY, Lee DH, Lee SB (2012) Tenacibaculum jejuense sp. nov., isolated from coastal seawater. Int J Syst Evol Microbiol 62:414–419
Park S, Park JM, Jung YT, Lee KC, Lee JS, Yoon JH (2014) Polaribacter marinivivus sp. nov., a member of the family Flavobacteriaceae isolated from seawater. Antonie Van Leeuwenhoek 106:1139–1146
Perry LB (1973) Gliding motility in some non-spreading flexibacteria. J Appl Microbiol 36:227–232
Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. MIDI Inc, Newark
Suzuki M, Nakagawa Y, Harayama S, Yamamoto S (2001) Phylogenetic analysis and taxonomic study of marine Cytophaga-like bacteria: proposal for Tenacibaculum gen. nov. with Tenacibaculum maritimum comb. nov. and Tenacibaculum ovolyticum comb. nov., and description of Tenacibaculum mesophilum sp. nov. and Tenacibaculum amylolyticum sp. nov. Int J Syst Evol Microbiol 51:1639–1652
Tamura K, Peterson D, Petersen N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using Maximum Likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739
Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882
Wang L, Li X, Hu D, Lai Q, Shao Z (2015) Tenacibaculum holothuriorum sp. nov., isolated from the sea cucumber Apostichopus japonicus intestine. Int J Syst Evol Microbiol doi: 10.1099/ijsem.0.000574
Weisburg WG, Barns SM, Pelletier DA, Lane DJ (1991) 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173:697–703
Worliczek HL, Kämpfer P, Rosengarten R, Tindall RBJ, Busse HJ (2007) Polar lipid and fatty acid profiles-re-vitalizing old approaches as a modern tool for the classification of mycoplasmas? Syst Appl Microbiol 30:355–370
Yoon JH, Lee MH, Jung YT (2013) Pseudofulvibacter geojedonensis gen. nov., sp. nov., a polysaccharide-degrading member of the family Flavobacteriaceae isolated from seawater, and emended description of the genus Fulvibacter. Int J Syst Evol Microbiol 63:16
We thank Professor Patricia Bergquist (University of Auckland, New Zealand) for taxonomic analysis of the sponge species. This work was supported by the New Energy and Industrial Technology Development Organization (NEDO), and in part by a research grant (2009–2011) of the Institute for Fermentation, Osaka, Japan.
Conflict of interest
The authors declare that they have no conflict of interest.
Electronic Supplementary Material
Below is the link to the electronic supplementary material.
A specimen of marine sponge Rhabdastrella sp. Supplementary material 1 (PPTX 83 kb)
Thin-layer chromatograms showing the total polar lipid compositions of A6F-119T. Total polar lipids were detected by spraying the plate with molybdophosphoric acid, molybdenun blue, α-naphthol, and ninhydrin. PE: phosphatidylenthanolamine, UAL: unidentified aminolipid, UL: unidentified lipid. Supplementary material 2 (PPTX 742 kb)
Negative results from the API 20E, API 50CH, and API ZYM strips. Supplementary material 3 (PPTX 44 kb)
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Yoon, J., Adachi, K. & Kasai, H. Spongiiferula fulva gen. nov., sp. nov., a Bacterium of the Family Flavobacteriaceae Isolated from a Marine Sponge. Curr Microbiol 73, 15–21 (2016). https://doi.org/10.1007/s00284-016-1022-z
- Marine Sponge
- Marine Agar
- Unidentified Lipid
- Polar Lipid Profile