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Effect of Lactobacillus Strains on Intestinal Microflora and Mucosa Immunity in Escherichia coli O157:H7-Induced Diarrhea in Mice

Abstract

This study investigated the effects of KLDS 1.8701 and AD1 administrations by gavage on intestinal microflora and mucosal immunity in diarrhea mice infected by Escherichia coli O157:H7 compared to normal mice. The levels of E. coli, Enterobacteria, and Enterococcus decreased significantly (P < 0.05), while viable counts of Lactobacilli and Bifidobacterium increased in diarrhea mice. Moreover, KLDS 1.8701 and AD1 improved secretion of secretory immunoglobulin A and enhanced the levels of interferon-γ and interleukin. Results indicate that KLDS 1.8701 and AD1 could effectively alleviate diarrhea in mice via modulation of intestinal microflora and improve the function of immune system. The study on the effect of KLDS1.8701 and AD1 supplementation in human flora-associated animal models was recommended.

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References

  1. Bian X, Huo G, Evivie SE, Muhammad Z, Luo GW, Liang HZ et al (2015) In vitro assessment of the antimicrobial potentials of Lactobacillus helveticus strains isolated from traditional cheese in Sinkiang China against food-borne pathogens. Food Funct 7(2):789–797

    Article  Google Scholar 

  2. Gálvez A, Abriouel H, López RL, Ben Omar N (2007) Bacteriocin-based strategies for food biopreservation. Int J Food Microbiol 120:51–70

    Article  PubMed  Google Scholar 

  3. Giraffa G (2014) Lactobacillus helveticus: importance in food and health. Front Microbiol 5(386):1–2

    Google Scholar 

  4. Kadariya J, Smith TC, Tapaliya D (2014) Staphlococcus aureus and Staphlococcal food-borne diseases: an ongoing challenge in public health. BioMed Res Int. doi:10.1155/2014/827965

    PubMed  PubMed Central  Google Scholar 

  5. Kidd P (2003) Th1/Th2 balance: the hypothesis, its limitations, and implications for health and disease. Altern Med Rev. 8:223–246

    PubMed  Google Scholar 

  6. Lee YE, Kang IJ, Yu EA, Kim S, Lee HJ (2012) Effect of feeding the combination with Lactobacillus plantarum and Bacillus subtilis on fecal microflora and diarrhea incidence of Korean native calves. Korean J Vet Serv 35:343–346

    Article  Google Scholar 

  7. López MC (2010) 5.10-mucosal immunity, comprehensive toxicology, vol 5, 2nd edn. American Chemical Society, Washington, pp 203–215

    Google Scholar 

  8. Maassen C, van Holten-Neelen C, Balk F, Heijne den Bak-Glashouwer MJ, Leer RJ, Laman JD, Boersma WJ, Claassen E (2000) Strain-dependent induction of cytokine profiles in the gut by orally-administered Lactobacillus strains. Vaccine 18:2613–2623

    CAS  Article  PubMed  Google Scholar 

  9. Mead PS, Griffin PM (1998) Escherichia coli O157:H7. Lancet 352:1207–1212

    CAS  Article  PubMed  Google Scholar 

  10. Michinaga O, Kensuke S, Koji N (2001) Protective effect of Lactobacillus casei strain Shirota on Shiga toxin-producing Escherichia coli O157:H7 infection in infant rabbits. Infect Immun 69:1101–1108

    Article  Google Scholar 

  11. Mosmann TR, Cherwinski H, Bond MW, Giedlin MA, Coffman RL (1986) Two types of murine helper T cell clone. I. Definition according to profiles of lymphokine activities and secreted proteins. J Immunol 136:2348–2357

    CAS  PubMed  Google Scholar 

  12. Nagashima K, Yasokawa D, Abe K, Nakagawa R, Kitamura T, Miura T (2010) Effect of a Lactobacillus species on incidence of Diarrhea in calves and change of the microflora associated with growth. Biosci Microflora 29:97–110

    CAS  Article  Google Scholar 

  13. Nakamura Y (2012) Upregulation of intestinal SIgA secretion by prebiotics and probiotics. Bifidus 26(1):3–9

    Google Scholar 

  14. Nataro JP, Kaper JB (1998) Diarrheagenic Escherichia coli. Clin Microbiol Rev 11:144–201

    Google Scholar 

  15. Oh YR, Lee JB, Park SY, Song CS, Choi IS, Kim YH (2008) In vivo evaluation of preventive effect of Lactobacillus reuteri on porcine epidemic diarrhea in suckling piglets. Korean J Vet Res 48:167–174

    Google Scholar 

  16. Perdigon G, Maldonado GC, Valdez JC, Medici M (2002) Interaction of lactic acid bacteria with the gut immune system. Eur J Clin Nutr 56:S21–S26

    CAS  Article  PubMed  Google Scholar 

  17. Riley LW, Remis RS, Helgerson SD et al (1983) Hemorrhagic colitis associated with a rare Escherichia coli Serotype. N Engl J Med 308:75–79

    Article  Google Scholar 

  18. Tachikawa T, Seo G, Nakazawa M, Sueyoshi M, Ohishi T, Joh K (1998) Estimation of probiotics by infection model of infant rabbit with enterohemorrhagic Escherichia coli O157:H7. Kansenshogaku Zasshi 72:1300–1305

    CAS  Article  PubMed  Google Scholar 

  19. The World Health Organisation (2015) Health topics: food-borne diseases. http://www.who.int/topics/foodborne_diseases/en/. Accessed 17 Apr 2015

  20. Tsai YT, Cheng PC, Pan TM (2012) The immunomodulatory effects of lactic acid bacteria for improving immune functions and benefits. Appl Microbiol Biotechnol 96:853–862

    CAS  Article  PubMed  Google Scholar 

  21. Wang YX, Chen QS (2008) Effects of Lactobacillus helveticus on intestinal microflora in mice. Food Sci 29:542–546 (in Chinese)

    Google Scholar 

  22. Yang Y, Yuan YJ, Tao YH, Wang W (2011) Effects of vitamin A deficiency on mucosal immunity and response to intestinal infection in rats. Nutrition 27:227–232

    CAS  Article  PubMed  Google Scholar 

  23. Zhang L, Xu YQ, Liu HY, Lai T, Ma JL, Wang JF, Zhu YH (2010) Evaluation of Lactobacillus rhamnosus GG using an Escherichia coli K88 model of piglet diarrhoea: effects on diarrhoea incidence, faecal microflora and immune responses. Vet Microbiol 141:142–148

    CAS  Article  PubMed  Google Scholar 

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Acknowledgments

This study was funded by 863 program of China:characteristic resources base of Lactic acid bacteria and research on lactic acid bacteria starter and metabolic engineering technology (2011AA100902) and Synergetic Innovation Center of Food Safety and Nutrition. The authors are also grateful for the financial support of the Ministry of Education, China.

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Correspondence to Gui-Cheng Huo.

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There was no conflict of interest in this study.

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All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. This article does not contain any studies with human participants performed by any of the authors.

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Bian, X., Wang, Tt., Xu, M. et al. Effect of Lactobacillus Strains on Intestinal Microflora and Mucosa Immunity in Escherichia coli O157:H7-Induced Diarrhea in Mice. Curr Microbiol 73, 65–70 (2016). https://doi.org/10.1007/s00284-016-1010-3

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Keywords

  • Lactobacillus
  • Viable Count
  • Lactobacillus Strain
  • Intestinal Microflora
  • Lactobacillus Acidophilus