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Rheinheimera gaetbuli sp. nov., a Marine Bacterium Isolated from a Tidal Flat

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Abstract

A gram-staining-negative, strictly aerobic, rod-shaped, and motile bacterium with a single polar flagellum, designated H26T, was isolated from tidal flat sediment in Jeju Island, South Korea. Growth of strain H26T was observed at 4–35 °C (optimum, 20–25 °C), pH 6.0–9.0 (optimum, pH 7.0–8.0), and 1–4 % NaCl (optimum, 2–3 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain H26T formed a phyletic lineage within the genus Rheinheimera, family Chromatiaceae. Strain H26T was most closely related to Rheinheimera baltica OSBAC1T, Rheinheimera aestuarii H29T, Rheinheimera muenzenbergensis E49T, and Rheinheimera aquimaris SW-353T with 98.5, 98.1, 97.8, and 97.5 % of 16S rRNA gene sequence similarities, respectively. The DNA–DNA relatedness levels between strain H26T and the type strains of R. baltica, R. aestuarii, R. muenzenbergensis, and R. aquimaris were 35.5 ± 3.2, 33.4 ± 1.5, 31.2 ± 2.2, and 28.7 ± 0.9 %, respectively. The major fatty acids of strain H26T were iso-C15:0 3-OH, summed feature 3 (comprising C16:1 ω7c/C16:1 ω6c), C16:0, summed feature 8 (comprising C18:1 ω7c/C18:1 ω6c), iso-C17:0 3-OH, and C12:0 3-OH and the strain contained ubiquinone (Q-8) as the sole isoprenoid quinone. Phosphatidylethanolamine, phosphatidylglycerol, and an aminolipid were identified as the major polar lipids and the G + C content of the genomic DNA was 52.0 mol%. Based on the phenotypic, chemotaxonomic, and molecular properties, strain H26T represents a novel species of the genus Rheinheimera, for which the name Rheinheimera gaetbuli sp. nov. is proposed. The type strain was H26T (=KACC 18254T = JCM 30403T).

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Acknowledgments

This work was supported by National Marine Biodiversity Institute of Korea (2015M00400) and the Program for Collection of Domestic Biological Resources from the National Institute of Biological Resources (NIBR No. 2015-02-001) of Ministry of Environment (MOE), Republic of Korea.

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Authors and Affiliations

  1. Department of Life Science, Chung-Ang University, Seoul, 06974, Republic of Korea

    Kyunghwa Baek & Che Ok Jeon

  2. National Marine Biodiversity Institute of Korea, Seocheon, Chungchungnam-do, 33662, Republic of Korea

    Kyunghwa Baek

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  1. Kyunghwa Baek
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  2. Che Ok Jeon
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Correspondence to Che Ok Jeon.

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Additional information

The GenBank accession number for the 16S rRNA gene sequence of strain H26T is KM588221.

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Baek, K., Jeon, C.O. Rheinheimera gaetbuli sp. nov., a Marine Bacterium Isolated from a Tidal Flat. Curr Microbiol 72, 344–350 (2016). https://doi.org/10.1007/s00284-015-0956-x

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