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Polyphasic Characterization of Lysobacter maris sp. nov., a Bacterium Isolated from Seawater

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Abstract

A strictly aerobic, Gram-negative, apricot-pigmented, non-motile, rod-shaped strain designated KMU-14T was isolated from seawater collected from the coastal zone of Yokji Island, Gyeongsangnam-do, Republic of Korea. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that the novel isolate was affiliated with the genus Lysobacter within the class Gammaproteobacteria and that it showed the highest sequence similarity (97.1 %) to Lysobacter concretionis Ko07T. The hybridization value for DNA–DNA relatedness between the strains of KMU-14T and L. concretionis Ko07T was 34.8 %, which was lower than 70 %, the recommended delineation value for differentiation of species. The DNA G+C content of strain KMU-14T was 64.9 mol%. The major respiratory quinone was ubiquinone 8 (Q-8), and iso-C15:0, iso-C16:0, and 10-methyl C16:0 and/or iso-C17:1 ω9c were the major (>10 %) cellular fatty acids. A polar lipid profile was present consisting of diphosphatidylglycerol, phosphatidylethanolamine, an unidentified phosphoglycolipid, two unidentified aminophospholipids, and two unidentified phospholipids. From the distinct phylogenetic position and combination of genotypic and phenotypic characteristics, the strain is considered to represent a novel species for which the name Lysobacter maris sp. nov. is proposed. The type strain of L. maris sp. nov. is KMU-14T (=KCTC 42381T =NBRC 110750T).

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  1. College of Pharmacy, Keimyung University, 1095 Dalgubeoldaero, Dalseo-Gu, Daegu, 704-701, Republic of Korea

    Jaewoo Yoon

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  1. Jaewoo Yoon
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Correspondence to Jaewoo Yoon.

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Supplementary Fig. 1

Transmission electron micrograph of a negatively stained cell of strain KMU-14T. Bar 1 μm. Supplementary material 1 (PPTX 660 kb)

Supplementary Fig. 2

Thin-layer chromatograms showing the total polar lipid composition of KMU-14T. Total polar lipids were detected by spraying the plate with molybdophosphoric acid, molybdenun blue, α-naphthol and ninhydrin. DPG diphosphatidylglycerol, PE phosphatidylenthanolamine, UPGL unidentified phosphoglycolipid, UAPL unidentified aminophospholipid, UPL unidentified phospholipid. Supplementary material 2 (PPTX 1118 kb)

Supplementary Fig. 3

Negative results from the API 20E, API 50CH and API ZYM strips. Supplementary material 3 (PPTX 72 kb)

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Yoon, J. Polyphasic Characterization of Lysobacter maris sp. nov., a Bacterium Isolated from Seawater. Curr Microbiol 72, 282–287 (2016). https://doi.org/10.1007/s00284-015-0949-9

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  • DOI: https://doi.org/10.1007/s00284-015-0949-9

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