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Rapid Identification of Methicillin-Resistant Staphylococcus aureus (MRSA) by the Vitek MS Saramis system

Current Microbiology volume 72, pages 29–32 (2016)Cite this article

Abstract

A rapid, sensitive, and accurate Vitek MS assay was developed to distinguish clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA) from clinical isolates of methicillin-sensitive Staphylococcus aureus (MSSA) by developing an in-house knowledgebase of SuperSpectra. Three unique peaks, including peaks at 2305.6 and 3007.3 Da specific to MRSA, and 6816.7 Da specific to MSSA, were selected for differentiating MRSA and MSSA. This assay accurately identified 84 and 91 % of clinical MRSA and MSSA strains out of the total 142 clinically acquired S. aureus strains that were tested. This method will greatly improve the efficiency of single clinical sample identification of MRSA, thereby facilitating a reduction in the transmission of MRSA in clinical settings.

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Acknowledgments

The authors thank Shi Chen, Yibo Shi, Guoxiong Li, Xiaoyan Wu, Sufei Yu, Huoyang Lv, Kang Liao, Yi Li, Hua Yu, Qing Wu, and Yunjian Hu for providing bacteria isolates. This work was supported by Science and Technology Department of Zhejiang Province (Grant No. 2014C33191).

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Affiliations

  1. Second Affiliated Hospital of Zhejiang University, Hangzhou, China

    Ying Fang, Xuan Wang, Danxia Gu & Rong Zhang

  2. College of Pharmacy Science, Zhejiang University of Technology, Hangzhou, China

    Weiguang Shan & Jiaping Li

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  1. Weiguang Shan
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  2. Jiaping Li
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  3. Ying Fang
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  4. Xuan Wang
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  5. Danxia Gu
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  6. Rong Zhang
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Correspondence to Rong Zhang.

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Shan, W., Li, J., Fang, Y. et al. Rapid Identification of Methicillin-Resistant Staphylococcus aureus (MRSA) by the Vitek MS Saramis system. Curr Microbiol 72, 29–32 (2016). https://doi.org/10.1007/s00284-015-0913-8

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  • DOI: https://doi.org/10.1007/s00284-015-0913-8

Keywords

  • Cefoxitin
  • mecA Gene
  • Automate Identification System
  • Unique Peak
  • MSSA Strain