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Quantitative Analysis of the Migration and Accumulation of Bacillus subtilis in Asparagus officinalis

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Abstract

Bacillus subtilis B96-II is a broad-spectrum biological control strain. It effectively suppresses soil-borne fungal diseases in vegetables. A green fluorescence protein (GFP) was expressed in B96-II to detect migration of B96-II into the root and stem of asparagus. The GFP-tagged B96-II (B96-II-GFP) strain exhibited bright green fluorescence under a fluorescence microscope. GFP was stable and had no apparent effects on the growth of the strain. Asparagus plants were planted in the soil inoculated with B96-II-GFP. Our results showed that B96-II-GFP was detected in both the root and stem 15, 30, and 45 days after the asparagus seedlings were planted. B96-II-GFP was also detected in leaves but at a lower concentration. The highest concentration was detected in 15 days, and the number of bacteria decreased subsequently irrespective of duration of growth or sampling period. The highest concentration of B96-II-GFP was present in the root base suggesting that the root base served as the hub of bacterial migration from the soil to the stem.

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Acknowledgments

This research was funded by Shanxi Provincial Natural Science Foundation for Youth under Grant [2007021037] and Shanxi Provincial Natural Science Foundation under Grant [2012011046-4].

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Authors and Affiliations

  1. Institute of Loess Plateau, Shanxi University, Taiyuan, 030006, People’s Republic of China

    Bian-Qing Hao

  2. Institute of Agro-Products Quality, Safety and Testing Technology, Shanxi Academy of Agricultural Sciences, Taiyuan, 030031, People’s Republic of China

    Bian-Qing Hao, Li-Ping Ma & Xiong-Wu Qiao

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  1. Bian-Qing Hao
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  2. Li-Ping Ma
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Correspondence to Xiong-Wu Qiao.

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Hao, BQ., Ma, LP. & Qiao, XW. Quantitative Analysis of the Migration and Accumulation of Bacillus subtilis in Asparagus officinalis . Curr Microbiol 71, 357–362 (2015). https://doi.org/10.1007/s00284-015-0865-z

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  • DOI: https://doi.org/10.1007/s00284-015-0865-z

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