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Halorientalis brevis sp. nov., Isolated from an Inland Salt Lake of China

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Abstract

Halophilic archaeal strain YC89T was isolated from Yuncheng salt lake in Shanxi, China. Cells from strain YC89T were short rods, lysed in distilled water, stained Gram-negative and formed red-pigmented colonies on agar plate. Strain YC89T was able to grow at 25–50 °C (optimum 37 °C), at 1.4–4.8 M NaCl (optimum 2.6–3.1 M), at 0–1.0 M MgCl2 (optimum 0.3 M) and at pH 6.0–9.5 (optimum pH 7.5). The major polar lipids are phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, sulfated mannosyl glucosyl diether and two unknown glycolipids. 16S rRNA gene analysis revealed that strain YC89T was phylogenetically related to Halorientalis persicus D108T (95.6 % nucleotide identity) and H. regularis TNN28T (95.3 % nucleotide identity). The rpoB′ gene similarities between strain YC89T and H. persicus IBRC-M 10043T and H. regularis TNN28T were 88.1 and 88.0 %, respectively. The DNA G+C content of strain YC89T was determined to be 61.3 mol%. The phenotypic, chemotaxonomic and phylogenetic properties suggested that strain YC89T (=CGMCC 1.12125T = JCM 18366T) represents a new species of Halorientalis, for which the name H. brevis sp. nov. is proposed.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 31370054), the 11th “Six Talents Peak” Project of Jiangsu Province (No. 2014-SWYY-021), the Qinglan Project of Jiangsu Province and a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD). We are grateful to Dr. M. A. Amoozegar for kindly providing strain Halorientalis persicus IBRC-M 10043T.

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Authors and Affiliations

  1. School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Jingkou District, Zhenjiang, 212013, People’s Republic of China

    Pan-Pan Yuan, Shuai Yin, Dong Han, Wen-Jiao Zhang & Heng-Lin Cui

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  1. Pan-Pan Yuan
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  2. Shuai Yin
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  3. Dong Han
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  5. Heng-Lin Cui
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Correspondence to Heng-Lin Cui.

Additional information

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene and rpoB ′ gene sequences of strain YC89T are JQ237120 and KJ913076, respectively.

Phase-contrast micrograph of strain YC89T, thin-layer chromatograms of strain YC89T and related members, neighbour-joining phylogenetic tree reconstructions based on 16S rRNA gene and rpoB′ gene sequences showing the relationships between strain YC89T and related members within the family Halobacteriaceae are available as supplementary materials.

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Yuan, PP., Yin, S., Han, D. et al. Halorientalis brevis sp. nov., Isolated from an Inland Salt Lake of China. Curr Microbiol 71, 382–386 (2015). https://doi.org/10.1007/s00284-015-0861-3

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  • DOI: https://doi.org/10.1007/s00284-015-0861-3

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