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Paenibacillus enshidis sp. nov., Isolated from the Nodules of Robinia pseudoacacia L.

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Abstract

A Gram-positive, motile, endospore-forming, rod-shaped bacterium, designated RP-207T, was isolated from the nodules of Robinia pseudoacacia L. plants planted in Enshi District, Hubei, PR China. Phylogenetic analyses based on the 16S rRNA gene sequence showed that the novel strain was affiliated to the genus Paenibacillus, with its closest relatives being Paenibacillus xylanilyticus XIL14T (95.6 %), Paenibacillus peoriae DSM8320T (95.3 %) and Paenibacillus polymyxa DSM 36T (95.3 %). The DNA G+C content was 47.0 mol %. DNA–DNA hybridization value between strain RP-207T and P. xylanilyticus XIL14T was 40.1 %. The diamino acid found in the cell wall peptidoglycan was meso-diaminopimelic. The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, an unidentified amino-phospholipid and an unknown phospholipid. The predominant menaquinone was menaquinone-7 (MK-7), and the major fatty acid was anteiso-C15:0 and C16:0. On the basis of its physiological and biochemical characteristics and the level of DNA–DNA hybridization, strain RP-207T is considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus enshidis sp. nov. is proposed. The type strain is RP-207T (=CCTCC AB 2013275T = KCTC 33519T).

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References

  1. Alvarado I, Phui A, Elekonich MM, Abel-Santos E (2013) Requirements for in vitro germination of Paenibacillus larvae spores. J Bacteriol 195(5):1005–1011

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  2. Barrow GI, Feltham RKA (1993) Cowan and steel’s manual for the identification of medical bacteria, 3rd edn. Cambridge University Press, Cambridge, p 331

    Book  Google Scholar 

  3. Chou JH, Chou YJ, Lin KY, Sheu SY, Sheu DS, Arun AB, Young CC, Chen WM (2007) Paenibacillus fonticola sp. nov., isolated from a warm spring. Int J Syst Evol Microbiol 57:1346–1350

    Article  CAS  PubMed  Google Scholar 

  4. Ding Y, Wang J, Liu Y, Chen S (2005) Isolation and identification of nitrogen-fixing bacilli from plant rhizosphere in Beijing region. J Appl Microbiol 99:1271–1281

    Article  CAS  PubMed  Google Scholar 

  5. Doetsch RN (1981) Determinative methods of light microscopy. In: Gerdhardt P, Murray RGE, Costilow RN, Nester EW, Wood WA, Krieg NR, Phillips GB (eds) Manual of methods for general bacteriology. American Society for Microbiology, Washington, DC, pp 21–33

    Google Scholar 

  6. Enright MR, McInerney JO, Griffin CT (2003) Characterization of endospore-forming bacteria associated with entomopathogenic nematodes, Heterorhabditis spp., and description of Paenibacillus nematophilus sp. nov. Int J Syst Evol Microbiol 53:435–441

    Article  CAS  PubMed  Google Scholar 

  7. Huang E, Yousef AE (2012) Draft genome sequence of Draft genome sequence of Paenibacillus polymyxa OSY-DF, which coproduces a lantibiotic, paenibacillin, and polymyxin E1. J Bacteriol 194:4739–4740

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  8. Komagata K, Suzuki K (1987) Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207

    Article  CAS  Google Scholar 

  9. Lim JM, Jeon CO, Lee JC, Xu LH, Jiang CL, Kim CJ (2006) Paenibacillus gansuensis sp. nov., isolated from desert soil of Gansu Province in China. Int J Syst Evol Microbiol 56:2131–2134

    Article  CAS  PubMed  Google Scholar 

  10. MIDI (2001) Sherlock microbial identification system operating manual, version 4.0. MIDI, Inc., Newark

    Google Scholar 

  11. Minnikin DE, O’Donnell AG, Goodfellow M, Alderson G, Athalye M, Schaal A, Parlett JH (1984) An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241

    Article  CAS  Google Scholar 

  12. Montes MJ, Mercade E, Bozal N, Guinea J (2004) Paenibacillus antarcticus sp. nov., a novel psychrotolerant organism from the Antarctic environment. Int J Syst Evol Microbiol 54:1521–1526

    Article  CAS  PubMed  Google Scholar 

  13. Murray RGE, Doetsch RN, Robinow F (1994) Determinative and cytological light microscopy. In: Gerhardt P, Murray RGE, Wood WA, Krieg NR (eds) Methods for general and molecular bacteriology. American Society for Microbiology, Washington, DC, pp 21–41

    Google Scholar 

  14. Rivas R, Mateos PF, Martınez-Molina E, Velazquez E (2005) Paenibacillus xylanilyticus sp. nov., an airborne xylanolytic bacterium. Int J Syst Evol Microbiol 55:405–408

    Article  CAS  PubMed  Google Scholar 

  15. Saha P, Mondal AK, Mayilraj S, Krishnamurthi S, Bhattacharya A, Chakrabarti T (2005) Paenibacillus assamensis sp. nov., a novel bacterium isolated from a warm spring in Assam, India. Int J Syst Evol Microbiol 55:2577–2581

    Article  CAS  PubMed  Google Scholar 

  16. Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor

    Google Scholar 

  17. Scheldeman P, Goossens K, Rodriguez-Diaz M, Pil A, Goris J, Herman L, De Vos P, Logan NA, Heyndrickx M (2004) Paenibacillus lactis sp. nov., isolated from raw and heat-treated milk. Int J Syst Evol Microbiol 54:885–891

    Article  CAS  PubMed  Google Scholar 

  18. Schleifer KH (1985) Analysis of the chemical composition and primary structure of murein. Methods Microbiol 18:123–156

    Article  CAS  Google Scholar 

  19. Shimoyama T, Johari NB, Tsuruya A, Nair A, Nakayama T (2014) Paenibacillus relictisesami sp. nov., isolated from sesame oil cake. Int J Syst Bacteriol 64(5):1534–1539

    Article  CAS  Google Scholar 

  20. Tamaoka J, Komagata K (1984) Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128

    Article  CAS  Google Scholar 

  21. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  22. Tanasupawat S, Thawai C, Yukphan P, Moonmangmee D, Itoh T, Adachi O, Yamada Y (2004) Gluconobacter thailandicus sp. nov., an acetic acid bacterium in the a-proteobacteria. J Gen Appl Microbiol 50:159–167

    Article  CAS  PubMed  Google Scholar 

  23. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  24. Ziemke F, Hofle MG, Lalucat J, Rossello-Mora R (1998) Reclassification of Shewanella putrefaciens Owen’s genomic group II as Shewanella baltica sp. nov. Int J Syst Bacteriol 48:179–186

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

This work was supported by the Natural Science Foundation of Hubei Province (2014CFB914), and the Fundamental Research Funds for the Central Universities, South-Central University for Nationalities (CZZ12005 and CZW14001).

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Authors and Affiliations

  1. College of Life Science, South-Central University for Nationalities, Wuhan, 430074, China

    Jie Yin, Donglan He, Xiaohua Li, Xiaobo Zeng, Mengyang Tian & Guojun Cheng

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  1. Jie Yin
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  2. Donglan He
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  3. Xiaohua Li
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  4. Xiaobo Zeng
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  5. Mengyang Tian
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  6. Guojun Cheng
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Correspondence to Guojun Cheng.

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Fig. S1

Polar lipid profile of strain RP-207T after two-dimensional thin-layer chromatogram. DPG, diphosphatidylglycerol; PE, phosphatidylethanolamine; PG, phosphatidylglycerol; PL, unidentified phospholipid; PN, unidentified amino-phospholipid. Supplementary material 1 (TIFF 1166 kb)

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Yin, J., He, D., Li, X. et al. Paenibacillus enshidis sp. nov., Isolated from the Nodules of Robinia pseudoacacia L.. Curr Microbiol 71, 321–325 (2015). https://doi.org/10.1007/s00284-015-0854-2

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