Abstract
A strictly aerobic, Gram stain-negative, yellowish-orange-pigmented, non-motile, rod-shaped strain designated YJ019T was isolated from marine sediment collected at Hwangwooji, a natural pond in Jeju island, Republic of Korea. Preliminary analysis based on the 16S rRNA gene sequence revealed that the novel isolate was affiliated with the family Flavobacteriaceae within the phylum Bacteroidetes and that it showed the highest sequence similarity (97.7 %) to Gramella gaetbulicola RA5-111T. The hybridization values for DNA–DNA relatedness between strain YJ019T and the type strains of the five validly described Gramella species were lower than 70 %, which is accepted as the phylogenetic definition of a novel species. The DNA G+C content of strain YJ019T was 38.4 mol%. The major menaquinone was MK-6, and iso-C15:0, iso-C17:0 3-OH and summed feature 3 (C16:1 ω7c and/or C16:1 ω6c) were the major (>10 %) cellular fatty acids. A complex polar lipid profile was present consisting of phosphatidylethanolamine, an unidentified aminophospholipid, two unidentified aminolipids and two unidentified lipids. From the distinct phylogenetic position and combination of genotypic and phenotypic characteristics, the strain is considered to represent a novel species for which the name Gramella lutea sp. nov. is proposed. The type strain of Gramella lutea sp. nov. is YJ019T (=KCTC 42382T = NBRC 110751T).
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This work was supported by the National Research Foundation of Korea Grants (NRF-2014R1A1A2057302), Korea.
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Supplementary Fig. 1
Thin-layer chromatograms showing the total polar lipid compositions of YJ019T. Total polar lipids were detected by spraying the plate with molybdophosphoric acid, molybdenun blue, α-naphthol and ninhydrin. PE: Phosphatidylethanolamine, UAPL: Unidentified aminophospholipid, UAL: Unidentified aminolipid, UL: Unidentified lipid. Supplementary material 1 (PPT 1551 kb)
Supplementary Fig. 2
All negative results from the API 20E, API 50CH and API ZYM strips. Supplementary material 2 (PPTX 75 kb)
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Yoon, J., Jo, Y., Kim, G.J. et al. Gramella lutea sp. nov., a Novel Species of the Family Flavobacteriaceae Isolated from Marine Sediment. Curr Microbiol 71, 252–258 (2015). https://doi.org/10.1007/s00284-015-0849-z
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DOI: https://doi.org/10.1007/s00284-015-0849-z