Abstract
Mycoplasma pneumoniae causes chronic respiratory disease in humans. Factors thought to be important for colonization include the ability of the mycoplasma to form a biofilm on epithelial surfaces and the production of hydrogen peroxide to damage host tissue. Almost all of the mycoplasmas, including M. pneumoniae, lack superoxide dismutase and catalase and a balance should exist between peroxide production and growth. We show here that the addition of catalase to cultures enhanced the formation of biofilms and altered the structure. The incorporation of catalase in agar increased the number of colony-forming units detected and hence could improve the clinical diagnosis of mycoplasmal diseases.
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References
Atkinson TP, Balish MF, Waites KB (2008) Epidemiology, clinical manifestations, pathogenesis and laboratory detection of Mycoplasma pneumoniae infections. FEMS Microbiol Rev 32:956–973
Waites KB, Talkington DF (2004) Mycoplasma pneumoniae and its role as a human pathogen. Clin Microbiol Rev 17:697–728
Principi N, Esposito S (2013) Macrolide-resistant Mycoplasma pneumoniae: its role in respiratory infection. J Antimicrob Chemother 68:506–511
Hames C, Halbedel S, Hoppert M, Frey J, Stülke J (2009) Glycerol metabolism is important for cytotoxicity of Mycoplasma pneumoniae. J Bacteriol 191:747–753
Cole BC, Ward JR, Martin CH (1968) Hemolysin and peroxide activity of Mycoplasma species. J Bacteriol 95:2023–2030
Miles RJ, Taylor RR, Varsani H (1991) Oxygen uptake and H2O2 production by fermentative Mycoplasma spp. J Med Microbiol 34:219–223
Linley E, Denyer SP, McDonnell G, Simons C, Maillard JY (2012) Use of hydrogen peroxide as a biocide: new consideration of its mechanisms of biocidal action. J Antimicrob Chemother 67:1589–1596
Brennan PC, Feinstein RN (1969) Relationship of hydrogen peroxide production by Mycoplasma pulmonis to virulence for catalase-deficient mice. J Bacteriol 98:1036–1040
Großhennig S, Schmidl SR, Schmeisky G, Busse J, Stülke J (2013) Implication of glycerol and phospholipid transporters in Mycoplasma pneumoniae growth and virulence. Infect Immun 81:896–904
Simmons WL, Daubenspeck JM, Osborne JD, Balish MF, Waites KB, Dybvig K (2013) Type 1 and type 2 strains of Mycoplasma pneumoniae form different biofilms. Microbiology 159:737–747
Simmons WL, Denison AM, Dybvig K (2004) Resistance of Mycoplasma pulmonis to complement lysis is dependent on the number of Vsa tandem repeats: shield hypothesis. Infect Immun 72:6846–6851
Simmons WL, Dybvig K (2007) Biofilms protect Mycoplasma pulmonis cells from lytic effects of complement and gramicidin. Infect Immun 75:3696–3699
Stewart PS, Roe F, Rayner J, Elkins JG, Lewandowski Z, Ochsner UA, Hassett DJ (2000) Effect of catalase on hydrogen peroxide penetration into Pseudomonas aeruginosa biofilms. Appl Environ Microbiol 66:836–838
Güell M, van Noort V, Yus E, Chen WH, Leigh-Bell J, Michalodimitrakis K, Yamada T, Arumugam M, Doerks T, Kühner S, Rode M, Suyama M, Schmidt S, Gavin AC, Bork P, Serrano L (2009) Transcriptome complexity in a genome-reduced bacterium. Science 326:1268–1271
Acknowledgments
We thank Portia Caldwell for the preparation of SP-4 medium. This work was supported by NIH Grant Number AI63909.
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The authors declare that they have no conflict.
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Simmons, W.L., Dybvig, K. Catalase Enhances Growth and Biofilm Production of Mycoplasma pneumoniae . Curr Microbiol 71, 190–194 (2015). https://doi.org/10.1007/s00284-015-0822-x
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DOI: https://doi.org/10.1007/s00284-015-0822-x