Abstract
A novel bacterial strain, designated Re6T, was isolated from forest soil collected in Campbell University, North Carolina. The cells are aerobic, Gram-positive, non-motile, and rod shaped. Growth occurred at 4–42 °C (optimum, 25 °C), pH 6–9 (optimum, pH 6), and in 0–3 % NaCl (w/v). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain Re6T belonged to the genus Leucobacter and showed the highest 16S rRNA gene sequence similarities with Leucobacter iarius JCM 14736T (98.3 %), Leucobacter luti JCM 14920T (97.9 %), Leucobacter komagatae JCM 9414T (97.8 %), and Leucobacter denitrificans KACC 14055T (97.7 %). Strain Re6T contained anteiso-C15:0 (45.2 %), iso-C16:0 (17.1 %), and anteiso-C17:0 (32.6 %) as the major cellular fatty acids; MK-11 as the major respiratory quinone; l-diaminobutyric acid as the diagnostic diamine acid in cell wall peptidoglycan; and diphosphatidylglycerol and phosphatidylglycerol as the main polar lipids. The DNA G+C content of the strain Re6T was 66.6 mol%. DNA–DNA hybridization results showed similarity values less than 50 % for DNA samples from the most closely related type strains of L. iarius, L. luti, and L. komagatae. On the basis of the data from the polyphasic analysis, strain Re6T is considered to be a representative novel species in the genus Leucobacter, for which the name Leucobacter humi sp. nov. is proposed. The type strain is Re6T (KEMC 7301-006T = JCM 18638T).
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Acknowledgments
This work was supported by Kyonggi University‘s Graduate Research Assistantship 2015, Korea national Environmental Microorganisms Bank (2010-0007473). This research was supported by a Grant (14CTAP-C078666-01) from infrastructure and transportation technology promotion research Program funded by Ministry of Land, Infrastructure, and Transport of Korean government.
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The NCBI GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain Re6T is KC818288.
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Her, J., Lee, SS. Leucobacter humi sp. nov., Isolated from Forest Soil. Curr Microbiol 71, 235–242 (2015). https://doi.org/10.1007/s00284-015-0820-z
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DOI: https://doi.org/10.1007/s00284-015-0820-z