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The Role of Plasma, Albumin, and Fibronectin in Staphylococcus epidermidis Adhesion to Polystyrene Surface

Current Microbiology volume 70, pages 846–853 (2015)Cite this article

Abstract

The influence of soluble and immobilized plasma, albumin, and fibronectin (Fn) on the adhesion of three Staphylococcus epidermidis strains to polystyrene was investigated. Both soluble and immobilized plasma and albumin cause to 7-fold reduction of the amounts of adhered cells, regardless of the strain used. The soluble Fn exhibited the adhesion for one strain and did not affect the bacterial sorption for remaining strains, whereas on Fn-coated polystyrene two of the three strains showed about 1.5-fold increase in the number of adsorbed bacteria. The plasma- and albumin-coated surfaces became much more hydrophilic as the contact angle changed from 78 ± 2° for control to 18 ± 2° for plasma and 21 ± 3° for albumin. The ligand–receptor specific interactions strains S. epidermidis with Fn-coated surfaces were proved by measuring the adhesion forces between cell surface and Fn-coated AFM tip. The surface roughness measured using AFM after the plasma and proteins immobilization was changed within 10 nm and not correlate with changes in bacterial adhesion.

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Acknowledgments

This work was supported by the RFBR under Grants 12-04-01431-a and 14-04-00687; and UB RAS under Grants 12-P-4-1002, 12-I-4-1003 and 12-M-14-2035.

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Authors and Affiliations

  1. Institute of Ecology and Genetics of Microorganisms UB RAS, Perm, Russia

    Daria Eroshenko & Vladimir Korobov

  2. Institute of Continuous Media Mechanics UB RAS, Perm, Russia

    Ilya Morozov

  3. Perm National State Research University, Perm, Russia

    Ilya Morozov

Authors
  1. Daria Eroshenko
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  2. Ilya Morozov
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  3. Vladimir Korobov
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Correspondence to Daria Eroshenko.

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Eroshenko, D., Morozov, I. & Korobov, V. The Role of Plasma, Albumin, and Fibronectin in Staphylococcus epidermidis Adhesion to Polystyrene Surface. Curr Microbiol 70, 846–853 (2015). https://doi.org/10.1007/s00284-015-0796-8

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  • DOI: https://doi.org/10.1007/s00284-015-0796-8

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