The Effect of CbbR-Binding Affinity to the Upstream of cbbF and cfxB on the Metabolic Effector in Rhodobacter sphaeroides

Abstract

Rhodobacter sphaeroides is a non-sulfur photosynthetic bacterium that possesses two cbb operons, cbb I and cbb II , encoding enzymes involved in the Calvin–Bensom–Bassham reductive pentose phosphate pathway of carbon dioxide fixation. In the present study, a number of molecules have been identified that have the ability to alter the in vivo DNA-binding properties of CbbR protein in R. sphaeroides. The CbbR-binding sites on the cbb operon in R. sphaeroides were characterized by chromatin immunoprecipitation (ChIP) assay. The ChIP assay indicated that the CbbR protein binds specifically to the upstream regions cbbF in cbb I operon and cfxB in cbb II operon. The change in the binding of CbbR to the upstream of cbbF and cfxB in the presence of RuBP, fructose 1,6-bisphosphate, NADPH, KH2PO4 was observed under anaerobic, aerobic, aerobic light–dark, and aerobic dark conditions, respectively. From these results, the role of different co-inducer molecules in influencing the interactions of CbbR with the binding site within cbb operon has been ascertained. The biosynthetic intermediates and other potential metabolic effectors have been observed to play an important role in the regulatory mechanism.

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Acknowledgments

This work was carried out with the support of “Cooperative Research Program for Agriculture Science & Technology Development (Project title: Development of microorganism of carbon dioxide reduction using waste medium and the bioplatic production from them, Project No: PJ01051502)” Rural Development Administration, Republic of Korea. The authors are grateful for their support.

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Correspondence to Yang-Hoon Kim or Jiho Min.

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Hyun Jeong Lee and Simranjeet Singh Sekhon have contributed equally to this work.

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Lee, H.J., Sekhon, S.S., Kim, Y.S. et al. The Effect of CbbR-Binding Affinity to the Upstream of cbbF and cfxB on the Metabolic Effector in Rhodobacter sphaeroides . Curr Microbiol 70, 816–820 (2015). https://doi.org/10.1007/s00284-015-0789-7

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Keywords

  • Calvin Cycle
  • ChIP Assay
  • Rhodobacter Sphaeroides
  • Rhodobacter Capsulatus
  • Rhodopseudomonas Palustris