Current Microbiology

, Volume 70, Issue 2, pp 195–198 | Cite as

Attachment of Escherichia coli to Listeria monocytogenes for Pediocin-Mediated Killing

  • Shanna Liu
  • Timo M. Takala
  • Justus Reunanen
  • Ossian Saris
  • Per E. J. SarisEmail author


Listeria phage endolysin cell wall-binding domain (CBD) from the Listeria phage A500 was fused with flagellar subunit FliC in Escherichia coli, aiming at binding of E. coli cells to Listeria cells, followed by enhanced killing of Listeria by pediocin production. FliC::CBD chimeric flagella were expressed and detected by Western blot. However, only few chimeric flagella could be isolated from the recombinant cells compared with sufficient amount of wild-type flagella obtained from the host cells. Interestingly, wild-type flagella extract showed capacity of binding Listeria cells. Pediocin-secreting E. coli cells with Listeria-binding flagella killed approximately 40 % of the Listeria cells, whereas cell-free spent growth medium with the same pediocin concentration only inhibited Listeria growth. These results suggested that binding the Listeria to bacteriocin-secreting cells improves killing.


Listeria Listeriosis Endolysin Protein Chimera Antilisterial Activity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This work was supported by China Scholarship Council, the University of Helsinki, Finland, and the Academy of Finland (project number 177321). Professor Martin Loessner is acknowledged for the kind gift of the Listeria strain and the CBD500 encoding DNA.


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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Shanna Liu
    • 1
    • 2
  • Timo M. Takala
    • 2
  • Justus Reunanen
    • 3
  • Ossian Saris
    • 2
  • Per E. J. Saris
    • 2
    Email author
  1. 1.College of Food Science and BioengineeringTianjin Agricultural UniversityTianjinPeople’s Republic of China
  2. 2.Department of Food and Environmental SciencesUniversity of HelsinkiHelsinkiFinland
  3. 3.Department of Veterinary BiosciencesUniversity of HelsinkiHelsinkiFinland

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