Potential Mode of Protection of Silkworm Pupae from Environmental Stress by Harboring the Bacterial Biofilm on the Surfaces of Silk Cocoons
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The silkworm forms cocoon to protect its pupa that survives for months inside the cocoon without being affected by various environmental stresses. To understand the possible mode of pupal survival within the cocoon encasement, we investigate the cause that protects the cocoon. During the end of the spinning process, we have isolated different bacterial species from the cocoon surface. These are identified using molecular techniques and checked for their abilities to form biofilm in vitro. The bacteria are able to form biofilm either individually or in consortia. Of which, Bacillus and Erwinia species are prominent biofilm formers. Interestingly, these bacteria have the ability to form biofilm on the cocoon mimetic surface of the silk protein Sericin Hope that contains only sericin. The origin and the behavior of the bacteria lead us to hypothesize the possible role of biofilm layer on the cocoon surface, which provides protection from adverse environmental conditions.
KeywordsBacterial Isolate Extracellular Polymeric Substance Stromatolite Calcium Oxalate Microbacterium
The work was supported in parts by three different grants. One from Department of Biotechnology (DBT) to SCK and two from Department of Biotechnology and North East (DBT-NE) Twinning grant and Council of Scientific and Industrial Research (CSIR) from Govt. of India to ASG.
Conflict of interest
The authors declare no commercial or financial conflict of interest.
- 14.Ralph WJ, John RT, Ray B (1995) Bacteriocins of Gram-positive bacteria. Microbiol Rev 59:171–200Google Scholar
- 15.Sasaki M, Yamada H, Osada K, Terada S (2010) Culturing animal cells using a medium containing sericin to produce proteins of interest; useful for producing animal cell growth stimulating agent: publication date Sep 28, 2010, Patent #US 7803616 B2Google Scholar
- 16.Sinohara H (1979) Glycopeptides isolated from sericin of the silkworm, Bombyx mori. Comp Biochem Physiol 6311:87–91Google Scholar
- 19.Wakimoto N, Nishi J, Sheikh J (2004) Quantitative biofilm assay using a microtiter plate to screen for enteroaggregative E. coli. Trop Med Hyg 71:687–690Google Scholar
- 20.Wingender J, Neu T, Flemming H (1999) What are bacterial extracellular polymeric substances?. In: (ed) Microbial extracellular polymeric substances, Springer Berlin Heidelberg, pp 1–19Google Scholar