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Acuticoccus yangtzensis gen. nov., sp. nov., a Novel Member in the Family Rhodobacteraceae, Isolated from the Surface Water of the Yangtze Estuary

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Abstract

A novel bacterium, strain JL1095T, was isolated from the surface water of the Yangtze Estuary, China (31° N, 122° E). Cells were Gram negative, aerobic, oval-shaped with one peak end and motile by gliding. Cells divided by binary fission. Growth occurred at 15–50 °C (optimum at 35 °C), 2–10 % (w/v) NaCl (optimum at ~3 %) and pH 6.0–9.0 (optimum at pH ~ 7.6). Strain JL1095T was able to utilize various sole-carbon-source, such as Tween 40, Tween 80, acetic acid, l-arabinose, d,l-lactic acid, urocanic acid, methyl-pyruvate, α-hydroxy butyric acid, β-hydroxy butyric acid, and γ-hydroxy butyric acid. The major cellular fatty acids were C16:0, C18:0, C19:0 ω8c cyclo, C20:1 ω7c, and Summed Feature 8. The whole respiratory ubiquinone was Q-10. The genomic DNA G+C content of strain JL1095T was 51.5 %. According to the phylogenetic analysis, strain JL1095T formed a monophyletic branch at the periphery of the evolutionary radiation occupied by the genera Labrenzia, Pannonibacter, Stappia, Wenxnia, and Amaricoccus. The sequence similarity was 92.8 % with the most closely relating strain Stappia indica B106T, and 92.6 % with the type species Stappia stellulatum IAM 12621T. Based on the biochemical characteristics, chemotaxonomy and phylogenetic analysis, strain JL1095T is considered to be a novel genus which belongs to the family Rhodobacteraceae. The novel strain is named Acuticoccus yangtzensis gen. nov., sp. nov. The type strain of Acuticoccus yangtzensis is JL1095T (=CGMCC 1.12795 = DSM 28604).

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Acknowledgments

The authors thank Dr. Susanne Verbarg, DSMZ Identification Service, Braunschweig, Germany, for determining chemotaxonomic properties of respiratory quinones and polar lipids. We also thank Qiliang Lai and Tianhua Zhong, Third Institute of Oceanography, State Oceanic Administration, for determination of fatty acid components and the genomic DNA G+C content. Keshao Liu, Wenchao Deng, and Yongle Xu were thanked for their assistance with the experiments. This work was supported by the OPWSR project 201105021, the MOST 973 program 2013CB955700, the NSFF project 2012J01182 and the NSFC projects 41176095, 91028001, 41121091, and 41023007.

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Authors and Affiliations

  1. State Key Laboratory of Marine Environmental Sciences, Xiamen University, Rd. Xiang’an Nan, Xiang’an District, Xiamen, 361101, Fujian, China

    Lei Hou, Yao Zhang, Jia Sun & Xiabing Xie

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  1. Lei Hou
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  2. Yao Zhang
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  3. Jia Sun
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  4. Xiabing Xie
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Corresponding author

Correspondence to Yao Zhang.

Additional information

The GenBank accession number for 16S rRNA gene sequence of strain JL1095T is KF741873.

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Hou, L., Zhang, Y., Sun, J. et al. Acuticoccus yangtzensis gen. nov., sp. nov., a Novel Member in the Family Rhodobacteraceae, Isolated from the Surface Water of the Yangtze Estuary. Curr Microbiol 70, 176–182 (2015). https://doi.org/10.1007/s00284-014-0698-1

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