Current Microbiology

, Volume 70, Issue 2, pp 151–153 | Cite as

Isolation and Characterization of a New Clostridium difficile Ribotype During a Prospective Study in a Hospital in Italy

  • Francesca SistoEmail author
  • Anna Maraschini
  • Giovanna Fabio
  • Serena Serafino
  • Miriam Zago
  • Maria Maddalena Scaltrito
  • Silvana Castaldi

Clostridium difficile is one of the most important agent of hospital infection with increasing rate of incidence [4]. During a prospective study conducted between 15th March 2011 and 15th March 2012, 142 C. difficile associated disease (CDAD) cases from four internal medicine wards of the Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico in Milano, were analyzed. The CDAD was confirmed by laboratory tests.

After alcolic shock, the isolates were recovered by inoculating a loopful of diarrheic stool samples onto C. difficile agar (CDA; Oxoid). They were identified with the rapid ID 32A system (bioMerieux) and toxins detected by the enzyme-linked immunosorbent assay (C. diff Quik Chek Complete™, Alere); toxigenic culture was also performed using the same enzyme-linked immunosorbent assay.

The isolates were typed by ribotyping [2]. The set of primer used were 16S and 23S, corresponding to bases 1482–1501 of the 16S ribosomal RNA gene of C. difficile(EMBL accession number...


Vancomycin Levofloxacin Imipenem Clostridium Difficile EUCAST 
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The authors wish to thank Dr. Patrizia Spigaglia (Istituto Superiore di Sanità, Rome) for performing capillary-sequencer-based PCR-ribotyping.


  1. 1.
    Barth H, Aktories K, Popoff MR, Siles GB (2004) Binary bacterial toxins: biochemistry, biology and application of common Clostridium and Bacillus proteins. Microbiol Mol Biol Rev 68:373–402PubMedCentralPubMedCrossRefGoogle Scholar
  2. 2.
    Bidet P, Barbut F, Lalande V, Burghoffer B, Petite JC (1999) Development of a new PCR-ribotyping method for Clostridium difficile based on ribosomal RNA gene sequencing. FEMS Microbiol Lett 175:261–266PubMedCrossRefGoogle Scholar
  3. 3.
    Laboratory Clinical Standards Institute (2013) Methods for antimicrobial susceptibility testing of anaerobic bacteria—Approved standard–Eight Edition, M11-A8. Clinical and Laboratory Standards Institute, WayneGoogle Scholar
  4. 4.
    Freeman J, Bauer MP, Baines SD, Corver J, Fawley WN, Goorhuis B et al (2010) The changing epidemiology of Clostridium difficile infections. Clin Microbiol Rev 23:529–549PubMedCentralPubMedCrossRefGoogle Scholar
  5. 5.
    Goncalves C, Decre D, Barbut F, Burghoffer B, Petit JC (2004) Prevalence and characterization of a binary toxin (actin-specific ADP-ribosyltransferase) from Clostridium difficile. J Clin Microbiol 42:1933–1939PubMedCentralPubMedCrossRefGoogle Scholar
  6. 6.
    Indra A, Huhulescu S, Schneeweis M, Hasenberger P, Kernbichler S, Fiedler A, Wewalka G, Allerberger F, Kuijper EJ (2008) Characterization of Clostridium difficile isolates using capillary gel electrophoresis-based PCR ribotyping. J Med Microbiol 57:1377–1382PubMedCentralPubMedCrossRefGoogle Scholar
  7. 7.
    Kato N, Ou CY, Bartley Kato H, SL Brown VK, Dowell VR, Ueno L (1991) Identification of toxigenic Clostridium difficile by the polymerase chain reaction. J Clin Microbiol 29:33–37PubMedCentralPubMedGoogle Scholar
  8. 8.
    Stubbs S, Rupnik M, Gibert M, Brazier J, Duerden B, Popoff M (2000) Production of actin-specific ADP-ribosyltransferase (binary toxin) by strains of Clostridium difficile. FEMS Microbiol Lett 186:307–312PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Francesca Sisto
    • 1
    Email author
  • Anna Maraschini
    • 2
  • Giovanna Fabio
    • 3
  • Serena Serafino
    • 3
  • Miriam Zago
    • 4
  • Maria Maddalena Scaltrito
    • 1
  • Silvana Castaldi
    • 5
  1. 1.Department of Biomedical, Surgical and Dental SciencesUniversity of MilanMilanItaly
  2. 2.UO Laboratorio Centrale di analisi chimico-cliniche e microbiologiaFondazione IRCCS Ca’ Granda Ospedale MaggioreMilanItaly
  3. 3.Department of Clinical Sciences and communityUniversità di Milano e Fondazione IRCCS Cà Granda PoliclinicoMilanItaly
  4. 4.Centro di Ricerca delle Produzioni Foraggere e Lattiero-CasearieLodiItaly
  5. 5.Department of Biomedical Sciences for HealthUniversità di Milano e Fondazione IRCCS Cà Granda PoliclinicoMilanItaly

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