Isolation and Characterization of a New Clostridium difficile Ribotype During a Prospective Study in a Hospital in Italy
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Clostridium difficile is one of the most important agent of hospital infection with increasing rate of incidence . During a prospective study conducted between 15th March 2011 and 15th March 2012, 142 C. difficile associated disease (CDAD) cases from four internal medicine wards of the Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico in Milano, were analyzed. The CDAD was confirmed by laboratory tests.
After alcolic shock, the isolates were recovered by inoculating a loopful of diarrheic stool samples onto C. difficile agar (CDA; Oxoid). They were identified with the rapid ID 32A system (bioMerieux) and toxins detected by the enzyme-linked immunosorbent assay (C. diff Quik Chek Complete™, Alere); toxigenic culture was also performed using the same enzyme-linked immunosorbent assay.
The isolates were typed by ribotyping . The set of primer used were 16S and 23S, corresponding to bases 1482–1501 of the 16S ribosomal RNA gene of C. difficile(EMBL accession number...
KeywordsVancomycin Levofloxacin Imipenem Clostridium Difficile EUCAST
The authors wish to thank Dr. Patrizia Spigaglia (Istituto Superiore di Sanità, Rome) for performing capillary-sequencer-based PCR-ribotyping.
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