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Planifilum caeni sp. nov., A Novel Member of Thermoactinomycete Isolated from Sludge Compost

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Abstract

A Gram positive, thermophilic, and filamentous bacterium, designated strain GIESS004T, was isolated from a sludge compost from Guangzhou, China. Growth occurred at 45–55 °C (optimum 50–53 °C) and pH 6.0–10.0 (optimum pH 7.0–7.5). The organism formed cream-yellow colonies with radial wrinkles. Aerial mycelium was not produced in any of the growth media tested. Single spores were produced along the substrate hyphae. Strain GIESS004T contained MK-7 as the predominant menaquinone and iso-C16:0, iso-C15:0 and iso-C17:0 as the major cellular fatty acids. The cell wall contained meso-diaminopimelic acid. The DNA G+C content was 60.7 mol%. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain GIESS004T was associated with the genus Planifilum and formed a cluster with its closest relative of Planifilum composti P8T (99.5 % similarity). However, the DNA–DNA pairing study showed that the isolate displayed reassociation value of only 57.6 ± 2.3 % with P. composti P8T. On the basis of the polyphasic taxonomic results, strain GIESS004T (= CCTCC AB2013107T = MCCC 1K00414T) is designated as the type strain of a novel species of the genus Planifilum, for which the name Planifilum caeni sp. nov. is proposed.

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Acknowledgments

This work was supported by the Agricultural Science & Technology Achievements Transfer Project of Guangdong Province, China (2012NL040), the Cooperation Project in Industry, Education & Research of Guangdong Province, China (2012B091100242) and the Foundation for Excellent Young Scientist in Guangdong Academy of Sciences (qnjj201401).

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Authors and Affiliations

  1. Guangdong Institute of Eco-environmental and Soil Sciences, Guangzhou, 510650, People’s Republic of China

    Zhen Yu, Chu Wu, Gui-Qin Yang & Shun-Gui Zhou

  2. Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou, 510640, People’s Republic of China

    Chu Wu, Gui-Qin Yang & Shun-Gui Zhou

  3. University of Chinese Academy of Sciences, Beijing, 100049, People’s Republic of China

    Chu Wu

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  1. Zhen Yu
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  2. Chu Wu
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  3. Gui-Qin Yang
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  4. Shun-Gui Zhou
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Corresponding author

Correspondence to Shun-Gui Zhou.

Additional information

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain GIESS004T is KF738257.

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Yu, Z., Wu, C., Yang, GQ. et al. Planifilum caeni sp. nov., A Novel Member of Thermoactinomycete Isolated from Sludge Compost. Curr Microbiol 70, 135–140 (2015). https://doi.org/10.1007/s00284-014-0693-6

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  • DOI: https://doi.org/10.1007/s00284-014-0693-6

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