Current Microbiology

, Volume 70, Issue 2, pp 169–175 | Cite as

Fusobacterium hwasookii sp. nov., Isolated from a Human Periodontitis Lesion

  • Eugene Cho
  • Soon-Nang Park
  • Yun Kyong Lim
  • Yeseul Shin
  • Jayoung Paek
  • Cheol Ho Hwang
  • Young-Hyo ChangEmail author
  • Joong-Ki KookEmail author


In this study, we classified the five strains (ChDC F128T, ChDC F145, ChDC F174, ChDC F206, and ChDC F300) as a novel species of genus Fusobacterium by DNA–DNA hybridization and multi-locus phylogenetic analysis (MLPA), based on a single sequence (24,715 bp) of 22 concatenated housekeeping genes, with morphological and chemotaxonomic characteristics. DNA–DNA hybridization data showed that the values of genomic relatedness between ChDC F128T and each of the other novel strains were ranged from 79.0 to 82.6 %, while those of genomic relatedness between ChDC F128T and type strain of each of subspecies of F. nucleatum or Fusobacterium periodonticum were ranged from 40.9 to 54.4 %. MLPA revealed that the 5 strains were clustered as one group and clearly discriminated with F. nucleatum and F. periodonticum with 100 % bootstrap value. The DNA G+C content of the five novel strains were ranged from 26.9 to 27.0 mol%. The cellular fatty acid analysis of clinical isolates and type strains revealed C14:0, C16:0, and cis-9 C16:1 as the major fatty acids. The cell wall peptidoglycan of the 5 strains was comprised of meso-lanthionine. These results show that the 5 strains are novel species and belong to the genus Fusobacterium. Strain ChDC F128T (=KCOM 1249T = KCTC 5108T = JCM 30218T) is suggested to be the type strain of a novel species of genus Fusobacterium, for which the name Fusobacterium hwasookii sp. nov. is proposed.


Major Fatty Acid Major Polar Lipid Subgingival Plaque KCTC 5108T Fusobacterium Nucleatum 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and future Planning (NRF-2012R1A1A2003324) and in part by a Grant TGM0721311 funded by the Ministry of Trade, Industry and Energy.

Supplementary material

284_2014_692_MOESM1_ESM.pptx (75 kb)
Supplementary Fig. S1. Neighbor-joining phylogenetic trees based on a single sequence (24,715 bp) by concatenating 22 housekeeping genes. The stability of phylogenetic trees was assessed by a bootstrap analysis of 1,000 replicates by using MEGA version 6.06 [17]. Bar indicates 0.01 changes per nucleotide position
284_2014_692_MOESM2_ESM.pptx (1.2 mb)
Supplementary Fig. S2. Scanning electron micrograph of strains (a) ChDC F128T, (b) ChDC F145, (c) ChDC F174, (d) ChDC F206, and (e) ChDC F300. The scale bars represent 30 μm (a), 10 μm (b), 100 μm (c), 20 μm (d), and 20 μm (e)
284_2014_692_MOESM3_ESM.pptx (875 kb)
Supplementary Fig. S3. Thin-layer chromatography of acid hydrolysates of peptidoglycan. Strains: 1, strain ChDC F128T; 2, F. nucleatum subsp. nucleatum ATCC25586T; 3, F. nucleatum subsp. polymorphum ATCC10953T; 4, F. nucleatum subsp. vincentii ATCC49256T; 5, F. nucleatum subsp. animalis ATCC51191T; 6, strain ChDC F128T; 7, F. periodonticum ATCC33693T. Arrow indicates meso-lanthionine (Lan)
284_2014_692_MOESM4_ESM.pptx (107 kb)
Supplementary Fig. S4. Polar lipid profile of ChDC F128T by two-dimensional thin-layer chromatography. Abbreviations: PE, Phosphatidylethanolamine; DPG, Diphosphatidylglycerol; PG, Phosphatidylglycerol; PL, unknown phospholipid; AL, unknown aminolipid; PN, unknown aminophospholipid; L, unknown polar lipid


  1. 1.
    Albandar JM, Brown LJ, Löe H (1997) Putative periodontal pathogens in subgingival plaque of young adults with and without early-onset periodontitis. J Periodontol 68:973–981PubMedCrossRefGoogle Scholar
  2. 2.
    Bolstad AI, Jensen HB, Bakken V (1996) Taxonomy, biology, and periodontal aspects of Fusobacterium nucleatum. Clin Microbiol Rev 9:55–71PubMedCentralPubMedGoogle Scholar
  3. 3.
    Dzink JL, Sheenan MT, Socransky SS (1990) Proposal of three subspecies of Fusobacterium nucleatum Knorr 1922: Fusobacterium nucleatum subsp. nucleatum subsp. nov., comb. nov.; Fusobacterium nucleatum subsp. polymorphum subsp. nov., nom. rev., comb. nov.; and Fusobacterium nucleatum subsp. vincentii subsp. nov., nom. rev., comb. nov. Int J Syst Bacteriol 40:74–78PubMedCrossRefGoogle Scholar
  4. 4.
    Ezaki T, Hashimoto Y, Yabuuchi E (1989) Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229. doi: 10.1099/00207713-39-3-224 CrossRefGoogle Scholar
  5. 5.
    Gharbia SE, Shah HN (1990) Heterogeneity within Fusobacterium nucleatum, proposal of four subspecies. Lett Appl Microbiol 10:105–108. doi: 10.1111/j.1472-765X.1990.tb00276.x CrossRefGoogle Scholar
  6. 6.
    Gharbia SE, Shah HN (1992) Fusobacterium nucleatum subsp. fusiforme subsp. nov. and Fusobacterium nucleatum subsp. animalis subsp. nov. as additional subspecies within Fusobacterium nucleatum. Int J Syst Bacteriol 42:296–298. doi: 10.1099/00207713-42-2-296 PubMedCrossRefGoogle Scholar
  7. 7.
    Jantzen E, Hofstad T (1981) Fatty acids of Fusobacterium species: taxonomic implications. J Gen Microbiol 123:163–171PubMedGoogle Scholar
  8. 8.
    Karpathy SE, Qin X, Gioia J, Jiang H, Liu Y, Petrosino JF, Yerrapragada S, Fox GE, Haake SK, Weinstock GM, Highlander SK (2007) Genome sequence of Fusobacterium nucleatum subspecies polymorphum: a genetically tractable fusobacterium. PLoS One 2:e659PubMedCentralPubMedCrossRefGoogle Scholar
  9. 9.
    Kato K, Umemoto T, Sagawa H, Kotani S (1979) Lanthionine as an essential constituent of cell wall peptidoglycan of Fusobacterium nucleatum. Curr Microbiol 3:147–151CrossRefGoogle Scholar
  10. 10.
    Kim HS, Lee DS, Chang YH, Kim MJ, Koh S, Kim J, Seong JH, Song SK, Shin HS, Son JB, Jung MY, Park SN, Yoo SY, Cho KW, Kim DK, Moon S, Kim D, Choi Y, Kim BO, Jang HS, Kim CS, Kim C, Choe SJ, Kook JK (2010) Application of rpoB and zinc protease gene for use in molecular discrimination of Fusobacterium nucleatum subspecies. J Clin Microbiol 48:545–553. doi: 10.1128/JCM.01631-09 PubMedCentralPubMedCrossRefGoogle Scholar
  11. 11.
    Könönen E, Kanervo A, Salminen K, Jousimies-Somer H (1999) Beta-lactamase production and antimicrobial susceptibility of oral heterogeneous Fusobacterium nucleatum populations in young children. Antimicrob Agents Chemother 43:1270–1273PubMedCentralPubMedGoogle Scholar
  12. 12.
    Kook JK, Park SN, Lim YK, Choi MH, Cho E, Kong SW, Shin Y, Paek J, Chang YH (2013) Fusobacterium nucleatum subsp. fusiforme Gharbia and Shah 1992 is a later synonym of Fusobacterium nucleatum subsp. vincentii Dzink et al. 1990. Curr Microbiol 66:414–417. doi: 10.1007/s00284-012-0289-y PubMedCrossRefGoogle Scholar
  13. 13.
    Moore WEC (1987) Microbiology of periodontal disease. J Perio Res 22:335–341CrossRefGoogle Scholar
  14. 14.
    Sadek F, Drucker DB, Boote V, Bennett KW, Eley A (1998) Phospholipids of Fusobacterium spp. J Appl Microbiol 85:302–308PubMedCrossRefGoogle Scholar
  15. 15.
    Slots J, Potts TV, Mashimo PA (1983) Fusobacterium periodonticum, a new species from the human oral cavity. J Dent Res 62:960–963PubMedCrossRefGoogle Scholar
  16. 16.
    Tamaoka J, Komagata K (1984) Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128. doi: 10.1111/j.1574-6968.1984.tb01388.x CrossRefGoogle Scholar
  17. 17.
    Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729. doi: 10.1093/molbev/mst197 PubMedCentralPubMedCrossRefGoogle Scholar
  18. 18.
    Tunér K, Baron EJ, Summanen P, Finegold SM (1992) Cellular fatty acids in Fusobacterium species as a tool for identification. J Clin Microbiol 30:3225–3229PubMedCentralPubMedGoogle Scholar
  19. 19.
    Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O, Krichevsky MI, Moore LH, Moore WEC, Murray RGE, Stackebrandt E, Starr MP, Truper HG (1987) Report of the Ad Hoc Committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464. doi: 10.1099/00207713-37-4-463 CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Eugene Cho
    • 1
  • Soon-Nang Park
    • 1
  • Yun Kyong Lim
    • 1
  • Yeseul Shin
    • 2
  • Jayoung Paek
    • 2
  • Cheol Ho Hwang
    • 3
  • Young-Hyo Chang
    • 2
    Email author
  • Joong-Ki Kook
    • 1
    Email author
  1. 1.Korean Collection for Oral Microbiology and Department of Oral Biochemistry, School of DentistryChosun UniversityGwangjuRepublic of Korea
  2. 2.Korean Collection for Type Cultures, Biological Resource CenterKRIBBDaejeonRepublic of Korea
  3. 3.Department of Environmental EngineeringChosun UniversityGwangjuRepublic of Korea

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