Abstract
A Gram-negative bacterial strain, designated WB1T, was isolated from a domestic refrigerator in Guangzhou, PR China. Cells of strain WB1T were oxidase-negative, catalase-positive, strictly aerobic, non-spore-forming and non-motile coccobacilli with peritrichous fimbriae-like structures. The strain was able to grow at 10–40 °C with optimum growth at 28–30 °C, pH 6.0–8.0 (optimum, pH 7.0) and 0–6 % NaCl (w/v, optimum, 0.5 %). Phylogenetic analyses based on 16S rRNA gene and rpoB gene sequences revealed that strain WB1T belonged to the genus Acinetobacter and was most closely related to A. indicus DSM 25388T (97.2 % 16S rRNA gene sequence similarity) and A. radioresistens NBRC 102413T (96.8 %). The DNA G + C content of strain WB1T was 46.74 ± 0.04 mol % and the major fatty acids comprised summed feature 3 (C16:1 ω7c and/or C16:1 ω6c), C18:1 ω9c, C16:0 and C12:0. The predominant respiratory quinone was identified as Q-9 and the polar lipids as diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine and an unidentified phospholipid. Phenotypic, phylogenetic and chemotaxonomic data, including low DNA–DNA relatedness with closely related type strains, supported that strain WB1T represents a distinct novel species in the genus Acinetobacter, for which the name Acinetobacter refrigeratorensis sp. nov. was proposed. The type strain is WB1T (=GIMCC 1.663T = CCTCC AB 2014197T = KCTC 42011T).
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Acknowledgments
We are grateful to Mrs. Ailing Zhang and Miss Zhiying Zhao in Guangdong Institute of Microbiology for help in the observation of cell morphology. This work was supported by the Natural Science Foundation of China (31170009), the Program of National Infrastructure of Microbial Resources [NIMR (2012)-01-02] and the National High Technology Research and Development Program of China (863 Program) (2014AA021501).
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Guangda Feng and Songzhen Yang have contributed equally to this study.
The GenBank/EMBL/DDBJ accession numbers for 16S rRNA gene and rpoB gene sequences of strain WB1T are KF709454 and KJ701022, respectively.
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Feng, G., Yang, S., Wang, Y. et al. Acinetobacter refrigeratorensis sp. nov., Isolated from a Domestic Refrigerator. Curr Microbiol 69, 888–893 (2014). https://doi.org/10.1007/s00284-014-0669-6
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DOI: https://doi.org/10.1007/s00284-014-0669-6