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Integration Host Factor is Required for the Induction of Acid Resistance in Escherichia coli

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Abstract

Integration host factor (IHF) is a heterodimeric histone-like DNA-binding protein that participates in many cellular functions. Many systems and global regulators of acid resistance (AR) under strongly acidic conditions have been reported, but the role of IHF has not been examined. In the present study, we report that IHF is necessary for the induction of AR in Escherichia coli. At acidic pH, a ∆ihfA∆ifhB-mutant strain was found to have significantly depressed levels of transcription of the arginine decarboxylase gene (adiA) and of translation of the lysine/cadaverine antiporter gene (cadB), when compared with wild-type strain. Thus, IHF induces the arginine- and lysine-dependent AR. These results indicate that in E. coli, by combined transcriptional and translational controls of gene expression, IHF activates expression of a specific set of genes required for survival at extremely acidic pH.

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Acknowledgments

The authors would like to thank Y. Kano and R. W. Simons for providing bacterial strains, and Dr. John Cronan for his valuable comments on the manuscript.

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  1. Department of Microbiology, University of Illinois, B103 Chemical and Life Sciences Laboratory, 601 South Goodwin Ave, Urbana, IL, 61801, USA

    Hongkai Bi & Changyi Zhang

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Correspondence to Hongkai Bi.

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Bi, H., Zhang, C. Integration Host Factor is Required for the Induction of Acid Resistance in Escherichia coli . Curr Microbiol 69, 218–224 (2014). https://doi.org/10.1007/s00284-014-0595-7

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