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Deinococcus soli sp. nov., a Gamma-Radiation-Resistant Bacterium Isolated from Rice Field Soil

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Abstract

A Gram-negative, non-motile, short rod-shaped bacterial strain, designated N5T, was isolated from a rice field soil in South Korea. Phylogenetic analysis based on the 16S rRNA gene sequence of the new isolate showed that strain N5T belongs to the genus Deinococcus, family Deinococcaceae, showing the highest sequence similarity to Deinococcus grandis KACC 11979T (98.4 %) and Deinococcus daejeonensis KCTC 13751T (97.5 %). Strain N5T exhibits resistance to gamma-radiation similar to that of other members of the genus Deinococcus, with a D10 value in excess of 4 kGy. Chemotaxonomic data showed that the most abundant fatty acids are C16:1 ω7c (25.25 %), C15:1 ω6c (19.77 %), C17:1 ω6c (11.87 %), and C17:0 (9.41 %), and the major polar lipid is an unknown phosphoglycolipid. The predominant respiratory quinone is menaquinone MK-8. The DNA G+C content is 71.4 mol%. Phenotypic, phylogenetic, and chemotaxonomic data support designation of strain N5T as a novel species of the genus Deinococcus, for which the name Deinococcus soli sp. nov. is proposed. The type strain is N5T (=KCTC 33153T = JCM 19176T).

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Acknowledgments

This work was supported by a special research grant from Seoul Women’s University (2014). We are grateful to Dr. Jean Euzéby for help with etymology, and to Dr. Sang-Yong Lim, and Mr. Min-Ho Joe in the Korea Atomic Energy Research Institute for their help with gamma-radiation-resistance testing.

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Authors and Affiliations

  1. Department of Life Science, Dongguk University-Seoul, Seoul, 100-715, Republic of Korea

    Seho Cha & Taegun Seo

  2. Department of Bio & Environmental Technology, College of Natural Science, Seoul Women’s University, Seoul, 139-774, Republic of Korea

    Sathiyaraj Srinivasan & Myung Kyum Kim

Authors
  1. Seho Cha
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  2. Sathiyaraj Srinivasan
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  3. Taegun Seo
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  4. Myung Kyum Kim
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Corresponding authors

Correspondence to Taegun Seo or Myung Kyum Kim.

Additional information

The NCBI GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain N5T (=KCTC 33153T=JCM 19176T) is KC771032.

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Cha, S., Srinivasan, S., Seo, T. et al. Deinococcus soli sp. nov., a Gamma-Radiation-Resistant Bacterium Isolated from Rice Field Soil. Curr Microbiol 68, 777–783 (2014). https://doi.org/10.1007/s00284-014-0542-7

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  • DOI: https://doi.org/10.1007/s00284-014-0542-7

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