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Current Microbiology

, Volume 68, Issue 6, pp 724–728 | Cite as

Dietary Supplementation of Usnic Acid, an Antimicrobial Compound in Lichens, Does Not Affect Rumen Bacterial Diversity or Density in Reindeer

  • Trine Glad
  • Perry Barboza
  • Roderick I. Mackie
  • André-Denis G. Wright
  • Lorenzo Brusetti
  • Svein D. Mathiesen
  • Monica A. Sundset
Article

Abstract

Reindeer (Rangifer tarandus tarandus) may include large proportions of lichens in their winter diet. These dietary lichens are rich in phenolic secondary compounds, the most well-known being the antimicrobial usnic acid. Previous studies have shown that reindeer host rumen bacteria resistant to usnic acid and that usnic acid is quickly detoxified in their rumen. In the present study, reindeer (n = 3) were sampled before, during, and after usnic acid supplementation to determine the effect on their rumen microbial ecology. Ad libitum intake of usnic acid averaged up to 278 mg/kg body mass. Population densities of rumen bacteria and methanogenic archaea determined by real-time PCR, ranged from 1.36 × 109 to 11.8 × 109 and 9.0 × 105 to 1.35 × 108 cells/g wet weight, respectively, and the two populations did not change significantly during usnic acid supplementation (repeated measures ANOVA) or vary significantly between the rumen liquid and particle fraction (paired t test). Rumen bacterial community structure determined by denaturing gradient gel electrophoresis did not change in response to intake of usnic acid. Firmicutes (38.7 %) and Bacteriodetes (27.4 %) were prevalent among the 16S rRNA gene sequences (n = 62) from the DGGE gels, but representatives of the phyla Verrucomicrobia (14.5 %) and Proteobacteria (1.6 %) were also detected. Rapid detoxification of the usnic acid or resistance to usnic acid may explain why the diversity of the dominant bacterial populations and the bacterial density in the reindeer rumen does not change during usnic acid supplementation.

Keywords

Firmicutes Bacteroidetes Usnic Acid Verrucomicrobia Rumen Bacterium 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This project is funded by The Reindeer Husbandry Research Fund as part of the International Polar Year consortium # 399 EALAT: Climate change and reindeer husbandry. We thank A. Falk, J. Edwards, A. Yannarell, and J.-N. Kim for technical assistance, and R. J. Forster, M. Morrison, and Dr. Zhongtang Yu for advice on the rumen sampling protocol.

Supplementary material

284_2014_534_MOESM1_ESM.docx (43 kb)
Supplementary material 1 (DOCX 42 kb)
284_2014_534_MOESM2_ESM.docx (47 kb)
Supplementary material 2 (DOCX 47 kb)

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Trine Glad
    • 1
  • Perry Barboza
    • 2
  • Roderick I. Mackie
    • 3
  • André-Denis G. Wright
    • 4
  • Lorenzo Brusetti
    • 5
  • Svein D. Mathiesen
    • 6
  • Monica A. Sundset
    • 1
  1. 1.Department of Arctic and Marine BiologyUiT The Arctic University of NorwayTromsøNorway
  2. 2.Institute of Arctic Biology and Department of Biology and WildlifeUniversity of AlaskaFairbanksUSA
  3. 3.Department of Animal SciencesUniversity of IllinoisUrbana-ChampaignUSA
  4. 4.Department of Animal ScienceUniversity of VermontBurlingtonUSA
  5. 5.Faculty of Science and TechnologyFree University of BozenBolzanoItaly
  6. 6.The Norwegian School of Veterinary ScienceTromsøNorway

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