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Description of Geodermatophilus amargosae sp. nov., to Accommodate the Not Validly Named Geodermatophilus obscurus subsp. amargosae (Luedemann, 1968)

An Erratum to this article was published on 01 March 2014

Abstract

A novel, gram reaction positive aerobic actinobacterium, designated G12T, not validly named as Geodermatophilus obscurus subsp. amargosae, was accessed in the DSMZ open collection as DSM 46136T. The optimal growth was at 2,535 °C, at pH 6.0–12.0 and in the absence of NaCl, forming greenish-black-coloured colonies on GYM agar. Chemotaxonomic and molecular characteristics of the isolate matched those described for members of the genus Geodermatophilus. The DNA G+C content of the strain was 73.0 mol%. The peptidoglycan contained meso-diaminopimelic acid as diagnostic diamino acid. The main phospholipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol and a small amount of phosphatidylglycerol; MK-9(H4) was the dominant menaquinone and galactose was detected as a diagnostic sugar. The major cellular fatty acid was branched-chain saturated acid iso-C15:0. The 16S rRNA gene showed 94.2–99.5 % sequence identity with the members of the genus Geodermatophilus. Based on the chemotaxonomic results and 16S rRNA gene sequence analysis, strain G12T is proposed to represent a novel species, Geodermatophilus amargosae. Type strain is G12T [=G96] (=DSM 46136T = CCUG 62971T = MTCC 11559T = ATCC 25081T = JCM 3153T = NBRC 13316T = NRRL B-3578T = KCTC 9360T). The INSDC accession number is HF679056.

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Acknowledgements

We would like to gratefully acknowledge the help of Bettina Sträubler and Birgit Grün for DNA–DNA hybridization analyses, Gabrielle Pötter for assistance with chemotaxonomy and Brian J. Tindall (all at DSMZ, Braunschweig) for his guidance in the chemotaxonomic analyses. M. C. Montero-Calasanz is the recipient of a postdoctoral contract from the European Social Fund Operational Programme (2007–2013) for Andalusia and a DSMZ postdoctoral fellowship.

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Correspondence to Maria del Carmen Montero-Calasanz.

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Montero-Calasanz, M.C., Göker, M., Rohde, M. et al. Description of Geodermatophilus amargosae sp. nov., to Accommodate the Not Validly Named Geodermatophilus obscurus subsp. amargosae (Luedemann, 1968). Curr Microbiol 68, 365–371 (2014). https://doi.org/10.1007/s00284-013-0479-2

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Keywords

  • Cellular Fatty Acid
  • Diamino Acid
  • DSMZ Medium
  • Potassium Tellurite
  • Mucic Acid