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Hymenobacter swuensis sp. nov., a Gamma-Radiation-Resistant Bacteria Isolated from Mountain Soil

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Abstract

Gram stain-negative and non-motile bacteria, designated as DY53T and DY43, were isolated from mountain soil in South Korea prior exposure with 5 kGy gamma radiation. Phylogenetic analysis based on 16S rRNA gene sequence revealed that the strains belonged to the family Cytophagaceae in the class Cytophagia. 16S rRNA gene sequence similarity of strains DY53T and DY43 was 100 %. The highest degrees of sequence similarities of strains DY53T and DY43 were found with Hymenobacter perfusus A1-12T (98.8 %), Hymenobacter rigui WPCB131T (98.5 %), H. yonginensis HMD1010T (97.9 %), H. xinjiangensis X2-1gT (96.6 %), and H. gelipurpurascens Txg1T (96.5 %). The DNA G+C content of the novel strains DY53T and DY43 were 59.5 mol%. Chemotaxonomic data revealed that strains possessed major fatty acids such as C15:0 iso, C15:0 anteiso, C16:1 ω5c, summed feature 3 (16:1 ω7c/ω6c), summed feature 4 (17:1 anteiso B/iso I) and C17:0 iso, and major polar lipid was phosphatidylethanolamine. The novel strains showed resistance to gamma radiation, with a D10 value (i.e., the dose required to reduce the bacterial population by tenfold) in excess of 5 kGy. Based on these data, strains DY53T and DY43 should be classified as representing a novel species, for which the name Hymenobacter swuensis sp. nov. is proposed, with the type strain DY53T (=KCTC 32018T = JCM 18582T) and DY43 (=KCTC 32010).

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Acknowledgments

We thank Dr. Jean Euzéby for checking the Latin etymology of species name and this work was supported by a special research grant from Seoul Women’s University (2013).

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Authors and Affiliations

  1. Department of Bio & Environmental Technology, College of Natural Science, Seoul Women’s University, Seoul, 139-774, Republic of Korea

    Jae-Jin Lee, Sathiyaraj Srinivasan, Sang Hee Lee, Shin Ae Kwon, Yoon Jung Kwon, Jin Lee, Jin Ju Choi, Hye Min Lee, Young Kyung Auh & Myung Kyum Kim

  2. Radiation Research Division for Biotechnology, Korea Atomic Energy Research Institute, Jeongeup, 580-185, Republic of Korea

    Sangyong Lim & Minho Joe

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  1. Jae-Jin Lee
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  2. Sathiyaraj Srinivasan
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  3. Sangyong Lim
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  4. Minho Joe
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  5. Sang Hee Lee
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  8. Jin Lee
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  10. Hye Min Lee
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Corresponding author

Correspondence to Myung Kyum Kim.

Additional information

The NCBI GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains DY53T and DY43 are JX308618 and KF255579.

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Lee, JJ., Srinivasan, S., Lim, S. et al. Hymenobacter swuensis sp. nov., a Gamma-Radiation-Resistant Bacteria Isolated from Mountain Soil. Curr Microbiol 68, 305–310 (2014). https://doi.org/10.1007/s00284-013-0478-3

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  • DOI: https://doi.org/10.1007/s00284-013-0478-3

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