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Improvement of Manganese Peroxidase Production by the Hyper Lignin-Degrading Fungus Phanerochaete sordida YK-624 by Recombinant Expression of the 5-Aminolevulinic Acid Synthase Gene

Current Microbiology volume 67, pages 708–711 (2013)Cite this article

Abstract

The manganese peroxidase (MnP) gene (mnp4) promoter of Phanerochaete sordida YK-624 was used to drive expression of 5-aminolevulinic acid synthase (als), which is a key heme biosynthesis enzyme. The expression plasmid pMnP4pro-als was transformed into P. sordida YK-624 uracil auxotrophic mutant UV-64, and 14 recombinant als expressing-transformants were generated. Average cumulative MnP activities in the transformants were 1.18-fold higher than that of control transformants. In particular, transformants A-14 and A-61 showed significantly higher MnP activity (approximately 2.8-fold) than wild type. RT-PCR analysis indicated that the increased MnP activity was caused by elevated recombinant als expression. These results suggest that the production of MnP is improved by high expression of als.

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Acknowledgments

This work was partially supported by Grants-in-Aid for Scientific Research (A) (Nos. 21248023 and 24248030) from the Ministry of Education, Culture, Sports, Science and Technology of Japan.

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Affiliations

  1. Graduate School of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka, 422-8529, Japan

    Hirofumi Hirai, Kenta Misumi & Hirokazu Kawagishi

  2. Research Institute of Green Science and Technology, Shizuoka University, Shizuoka, 422-8529, Japan

    Hirofumi Hirai, Tomohiro Suzuki & Hirokazu Kawagishi

  3. Graduate School of Science and Technology, Shizuoka University, Shizuoka, 422-8529, Japan

    Hirokazu Kawagishi

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  1. Hirofumi Hirai
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  2. Kenta Misumi
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  3. Tomohiro Suzuki
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  4. Hirokazu Kawagishi
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Correspondence to Hirofumi Hirai.

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Hirai, H., Misumi, K., Suzuki, T. et al. Improvement of Manganese Peroxidase Production by the Hyper Lignin-Degrading Fungus Phanerochaete sordida YK-624 by Recombinant Expression of the 5-Aminolevulinic Acid Synthase Gene. Curr Microbiol 67, 708–711 (2013). https://doi.org/10.1007/s00284-013-0428-0

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  • DOI: https://doi.org/10.1007/s00284-013-0428-0

Keywords

  • Enhanced Green Fluorescent Protein
  • Manganese Peroxidase
  • Versatile Peroxidase
  • Control Transformants
  • Phanerochaete Sordida