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Serological Cross-Reaction Between O-Antigens of Shigella dysenteriae Type 4 and an Environmental Escherichia albertii Isolate

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Abstract

An environmental freshwater bacterial isolate, DM104, appearing as Shigella-like colonies on selective agar plates was found to show strong and specific serological cross-reactivity with Shigella dysenteriae type 4. Biochemical identification according to the analytical profile index, molecular serotyping by restriction of the amplified O-antigen gene cluster (rfb-RFLP), together with phylogenetic analysis of the 16S rRNA gene and multi-locus sequence analysis, identified the isolate as Escherichia albertii. rfb-RFLP of DM104, revealed a profile different from that of S. dysenteriae type 4. However, western blot analysis of extracted lipopolysaccharides demonstrated strong cross-reactivity with S. dysenteriae type 4 using specific monovalent antisera and a lipopolysaccharide gel banding profile similar to that of S. dysenteriae type 4. The observed O-antigen cross-reaction between an E. albertii isolate and S. dysenteriae extends our knowledge of the extent of O-antigen cross-reaction within the Escherichia/Shigella group of organisms, and offers the possibility of using DM104 and similar cross-reacting strains as shigellosis vaccine candidates.

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Acknowledgments

This study was supported by The Norwegian Programme for Development, Research and Higher Education (NUFU) (Grant No. 2007/10063).

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Author notes

  1. Nafisa Azmuda

    Present address: Department of Microbiology, Jahangirnagar University, Savar, Dhaka, 1312, Bangladesh

  2. Selina Akter

    Present address: Department of Microbiology, Jessore Science and Technology University, Jessore, 7408, Bangladesh

Authors and Affiliations

  1. Department of Microbiology, University of Dhaka, Dhaka, 1000, Bangladesh

    Mohammed Ziaur Rahman, Selina Akter, Nafisa Azmuda, Munawar Sultana & Sirajul Islam Khan

  2. Department of Biology, University of Bergen, P.O. Box 7803, 5020, Bergen, Norway

    Mohammed Ziaur Rahman & Nils-Kåre Birkeland

  3. Centre for Health and Population Research, icddr,b, GPO Box 128, Dhaka, 1000, Bangladesh

    Mohammed Ziaur Rahman

  4. Institute Pasteur, Unite Biodiversité des Bactéries Pathogènes Emergentes, Paris, France

    Mohammed Ziaur Rahman, François-Xavier Weill & Patrick A. D. Grimont

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Correspondence to Nils-Kåre Birkeland.

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Rahman, M.Z., Akter, S., Azmuda, N. et al. Serological Cross-Reaction Between O-Antigens of Shigella dysenteriae Type 4 and an Environmental Escherichia albertii Isolate. Curr Microbiol 67, 590–595 (2013). https://doi.org/10.1007/s00284-013-0405-7

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  • DOI: https://doi.org/10.1007/s00284-013-0405-7

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