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A Novel Degenerated Primer Pair Detects Diverse Genes of Acyl Homoserine Lactone Synthetase in Rhizobiaceae Family

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Abstract

A novel degenerated primer set was designed to amplify acyl homoserine lactone (AHL) synthetase genes from members of the family Rhizobiaceae. The primer set successfully amplified AHL synthetase genes from pure cultures of AHL producers from Rhizobiaceae, but not from AHL producers out of the Rhizobiaceae family, indicating the specificity of this primer set to the Rhizobiaceae family. An inoculation experiment showed that the minimal detectable concentration of AHL producers from the soil was around 2.5 × 107 CFU/g soil. When applying to environmental samples, 7 and 14 different genotypes of AHL synthetase genes were identified in the rhizosphere of Glycine max and Vigna unguiculata, respectively, which revealed complicated and unknown AHL-based quorum-sensing networks in the rhizosphere. This is the first primer set that covers diverse AHL synthetase genes from different genera. It will be a useful culture-independent approach for better understanding of the ecological significance of QS in natural habitats.

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Acknowledgments

We would like to thank Prof Lizhong Zhu and Prof Baoliang Chen for their technical support and constructive discussion during the experiments. This work was financially supported by national natural science foundation of China (21137003, 21077090) and natural science foundation of Zhejiang Province (Y5100048) to Yili Huang.

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Authors and Affiliations

  1. Zhejiang Provincial Key Laboratory of Organic Pollution Process and Control, College of Environmental and Resource Sciences, Zhejiang University, Room B470, 688 Yu Hang Tang Road, Hangzhou, 310058, China

    Yili Huang & Yanhua Zeng

  2. College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, China

    Zhiliang Yu

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  1. Yili Huang
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  2. Yanhua Zeng
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  3. Zhiliang Yu
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Correspondence to Yili Huang.

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Huang, Y., Zeng, Y. & Yu, Z. A Novel Degenerated Primer Pair Detects Diverse Genes of Acyl Homoserine Lactone Synthetase in Rhizobiaceae Family. Curr Microbiol 67, 183–187 (2013). https://doi.org/10.1007/s00284-013-0351-4

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  • DOI: https://doi.org/10.1007/s00284-013-0351-4

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