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Immunochemical Characterization of the Capsular Polysaccharide of Azospirillum irakense KBC1

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Abstract

The repeating unit structure of Azospirillum irakense KBC1 capsular polysaccharide (CPS) was established and was found to be identical to that of the O polysaccharide of A. irakense KBC1 lipopolysaccharide (LPS). The antigenic heterogeneity of the LPS and the CPS was shown to be related to differences in the macromolecular organization of these glycopolymers. After an immune response activation, R-form CPS molecules were found to be predominant.

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Acknowledgments

We thank Professor Alexander S. Shashkov for his help with NMR spectroscopy and Mr. Dmitry N. Tychinin (IBPPM RAS, Saratov) for his help with translating the original manuscript into English. This study was supported by the Russian Foundation for Basic Research (Project no. 11-04-00533-a).

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Authors and Affiliations

  1. Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences (IBPPM RAS), 13 Prospekt Entuziastov, Saratov, 410049, Russia

    Yulia P. Fedonenko, Gennady L. Burygin, Irina A. Popova, Elena N. Sigida, Alina K. Surkina & Svetlana A. Konnova

  2. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 47 Leninsky Prospekt, Moscow, 119991, Russia

    Evelina L. Zdorovenko

  3. Chernyshevsky Saratov State University, 83 Ulitsa Astrakhanskaya, Saratov, 410012, Russia

    Svetlana A. Konnova

Authors
  1. Yulia P. Fedonenko
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  2. Gennady L. Burygin
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  3. Irina A. Popova
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  4. Elena N. Sigida
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  5. Alina K. Surkina
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  6. Evelina L. Zdorovenko
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  7. Svetlana A. Konnova
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Correspondence to Yulia P. Fedonenko.

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Fedonenko, Y.P., Burygin, G.L., Popova, I.A. et al. Immunochemical Characterization of the Capsular Polysaccharide of Azospirillum irakense KBC1. Curr Microbiol 67, 234–239 (2013). https://doi.org/10.1007/s00284-013-0346-1

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  • DOI: https://doi.org/10.1007/s00284-013-0346-1

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