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Changes in Membrane Fatty Acid Composition of Pseudomonas aeruginosa in Response to UV-C Radiations

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Abstract

The changes in lipid composition enable the micro-organisms to maintain membrane functions in the face of environmental fluctuations. The relationship between membrane fatty acid composition and UV-C stress was determined for mid-exponential phase and stationary phase Pseudomonas aeruginosa. The total lipids were obtained by dichloromethane/methanol (3:1) and were quantified by GC. The TLC analysis of phospholipids showed the presence of three major fractions phosphatidylethanolamine, phosphatidylglycerol, and cardiolipin. Significant modifications, as manifested by an increase of UFA, were obtained. Interestingly, this microorganism showed a remarkable capacity for recovery from the stressful effects of UV-C.

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Authors and Affiliations

  1. Laboratoire de Traitement des Eaux Usées, Centre des Recherches et des Technologies des Eaux (CERTE), Technopole Borj Cédria, P.O. Box 273, 8020, Soliman, Tunisia

    Salma Kloula Ben Ghorbal, Abdelwaheb Chatti, Lobna Maalej, Sana Kefacha & Abdennaceur Hassen

  2. Laboratoire de biochimie, Hôpital La Rabta, Tunis, Tunisia

    Mohamed Marwan Sethom & Moncef Feki

  3. Unité de Biochimie des Lipides et interaction des macromolécules en Biologie, 03/UR/0902, Laboratoire de Biochimie et Biologie Moléculaire, Faculté des Sciences de Bizerte, Zarzouna, Université de Carthage, 7021, Tunis, Tunisia

    Mouadh Mihoub & Ahmed Landoulsi

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  1. Salma Kloula Ben Ghorbal
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  2. Abdelwaheb Chatti
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  3. Mohamed Marwan Sethom
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  4. Lobna Maalej
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  5. Mouadh Mihoub
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  6. Sana Kefacha
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  7. Moncef Feki
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  9. Abdennaceur Hassen
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Correspondence to Abdelwaheb Chatti.

Additional information

Salma Kloula Ben Ghorbal and Abdelwaheb Chatti have contributed equally in this study.

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Ghorbal, S.K.B., Chatti, A., Sethom, M.M. et al. Changes in Membrane Fatty Acid Composition of Pseudomonas aeruginosa in Response to UV-C Radiations. Curr Microbiol 67, 112–117 (2013). https://doi.org/10.1007/s00284-013-0342-5

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  • DOI: https://doi.org/10.1007/s00284-013-0342-5

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