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Molecular Typing of Paenibacillus larvae Strains Isolated from Bulgarian Apiaries Based on Repetitive Element Polymerase Chain Reaction (Rep-PCR)

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Abstract

The aim of the present study was to perform molecular typing of Paenibacillus larvae (P. larvae) isolates from Bulgarian apiaries with repetitive element polymerase chain reaction (rep-PCR) using BOX A1R, MBO REP1, and ERIC primers. A total of 96 isolates collected from brood combs with clinical symptoms of American foulbrood originating from apiaries located in different geographical regions of Bulgaria, a reference strain P. larvae NBIMCC 8478 and 30 commercial honey samples with Bulgarian origin were included in the study. Rep-PCR fingerprinting analysis revealed two genotypes ab and AB of P. larvae isolates from brood combs and honey samples. A combination of genotypes ab/AB was detected in one apiary and honey sample. The prevailing genotype ab was found in 78.1 % of brood combs isolates as well as in the reference strain whereas genotype AB was determined in 21.9 % of isolates. The examination of honey samples confirmed the preponderance of ab genotype which was demonstrated in 20 of 30 samples analyzed. In conclusion, the genetic epidemiology of P. larvae revealed two genotypes—ab and AB for Bulgarian strains. Developed protocols for molecular typing of P. larvae are reliable and may be used to trace the source of infection.

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Acknowledgment

The research was funded by the projects 4/2009 and 4/2012 at Trakia university, Faculty of Veterinary Medicine, Stara Zagora, Bulgaria.

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Authors and Affiliations

  1. Department of Veterinary Microbiology, Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, Trakia University, 6000, Stara Zagora, Bulgaria

    Nikolina Rusenova & Parvan Parvanov

  2. Department of Molecular Biology, Faculty of Medicine, Trakia University, 6000, Stara Zagora, Bulgaria

    Spaska Stanilova

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  1. Nikolina Rusenova
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  2. Parvan Parvanov
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  3. Spaska Stanilova
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Correspondence to Nikolina Rusenova.

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Rusenova, N., Parvanov, P. & Stanilova, S. Molecular Typing of Paenibacillus larvae Strains Isolated from Bulgarian Apiaries Based on Repetitive Element Polymerase Chain Reaction (Rep-PCR). Curr Microbiol 66, 573–577 (2013). https://doi.org/10.1007/s00284-013-0318-5

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  • DOI: https://doi.org/10.1007/s00284-013-0318-5

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